Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1977-10-11
1979-09-25
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C12D 1306
Patent
active
041690117
ABSTRACT:
3'-Phosphoadenosine 5'-phosphosulfate, also known as PAPS, is useful in establishing sulfate transfer mechanisms in animals and may be produced by a chemical process yielding 68-72% product from a pure adenosine 2',3'-cyclic phosphate 5'-phosphate, which compound is initially prepared from the reaction of adenosine and pyrophosphoryl chloride. In the present procedure the pure cyclic phosphate is reacted with triethylamine-N-sulfonic acid to produce 2',3'-cyclic phosphate 5'-phosphosulfate. Subsequently, by hydrolysis with the enzyme ribonuclease-T.sub.2, the desired compound is produced. Alternatively, the 2'-phosphoadenosine 5'-phosphosulfate, known as iso-PAPS, may be produced from 2',3'-cyclic phosphate 5'-phosphosulfate by treatment with a different enzyme, PDase or spleen phosphodiesterase. This latter compound, iso-PAPS, biologically has only one-third the activity of PAPS, the natural isomer.
REFERENCES:
patent: 3268416 (1966-08-01), Igarasi et al.
Cherniak, et al., J. Biol. Chem. 239:2986-2990 (1964).
Simonscits, et al., Biochim. Biophys. Acta 395:74-79 (1975).
Rozhin et al., J. Biol. Chem. 249:2079-2087 (1974).
Horwitz et al., Biochim. Biophys. Acta, 480:376-381 (1977).
Horwitz Jerome P.
Huo Anne L.
Misra Radhey S.
Neenan John P.
Philips, deceased Kirsten D.
Roberts, Jr. John S.
Tanenholtz Alvin E.
The United States of America as represented by the Department of
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