Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-10-23
2000-02-15
Brusca, John S.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 4353201, 435476, 435488, 536 231, 536 241, C12Q 168, C12P 1934, C12N 1574, C07H 2104
Patent
active
06025131&
ABSTRACT:
A new method for the identification of useful promoters is disclosed. The method is capable of identifying bacterial promoters sensitive to a particular cellular insult and may be modified to identify promoters sensitive to herbicides and crop protection chemicals. Constructs comprising promoters upstream of a luminescent reporter genes are placed in transformed hosts. Transformants grown in liquid media to a predetermined growth stage and contacted with a cellular insult are assessed for regulatory region activity by measurement of the resulting change in bioluminesence. The method is able to identify promoters undetectable by standard methods.
REFERENCES:
patent: 5595896 (1997-01-01), Coruzzi et al.
McCann et al., An evaluation of Salmonella (Ames) test data in the published literature: Application of statistical procedures and analysis of mutagenic potency, Mutat. Res., 134, pp: 1-47 (1984).
Orser et al., Use of Prokaryotic Stress Promoters as Indicators of the Mechanisms of Chemical Toxicity, (In Vitro Toxicol., 8(1):71-85) Mary Ann Liebert, Inc., Publishers (1995).
Burlage et al., Monitoring of Naphthalene Catabolism by Bioluminescence with nah-lux Transcriptional Fusions, J. Bacteriology, 172(9):4749-4757 (1990).
Szittner and Meighen, Nucleotide Sequence, Expression, and Properties of Luciferase Coded by lux Genes from a Terrestrial Bacterium, J. Biol. Chem., 265:16581-16587 (1990).
Rupani et al., Characterization of the Stress Response of a Bioluminescent Biological Sensor in Batch and Continuous Cultures, Biotechnol Prog, 12:387-392 (1996).
Hill et al., Review The application of lux genes, Biotechnol. Appl. Biochem. 17:3-14 (1993).
Tatsumi et al., Molecular cloning and expression in Escherichia coli of a cDNA clone encoding luciferase of a firefly, Luciola lateralis, Biochem. Biophysica Acta. 1131:161-165 (1992).
Jacobs et al., Highly bioluminescent Bacillus subtilis obtained through high-level expression of a luxAB fusion gene, Mol. Gen. Genet., 230:251-256 (1991).
LaRossa et al., Physiological roles of the DnaK and GroE stress protein: catalysts of protein folding or macromolecular sponges? Mol. Microbiology, 5(3):529-534 (1991).
VanBogelen et al. Differential Induction of Heat Shock, SOS, and Oxidation Stress Regulons and Accumulation of Nucleotides in Escherichia coli, ( J. Bacteriology, 169(1):26-32 (1987).
Kenyon and Walker, DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli, Proc. Natl. Acad. Sci. U.S.A., 577(5):2819-2823 (1980).
Blom et al., Unique and Overlapping Pollutant Stress Proteins of Escherichia coli, Appl. Environ. Microbiol., 58(1):331-334 (1992).
Simons et al., Improved single and multicopy lac-based cloning vectors for protein and operon fusions Gene, Elsevier Science Publishers B.V. GEN 01960, 53:85-96 (1987).
Engebrecht et al., Measuring Gene Expression with Light, Science, 227:1345-1347 (1985).
Carmi, O.A., et al., Use of Bacterial Luciferase to Establish a Promoter Probe Vehicle Capable of Nondestructive Real-Time Analysis of Gene Expression in Bacillus spp., J. Bacteriol., 169(5):2165-2170 (1987).
Guzzo and DuBow, Construction of stable, single-copy luciferase gene fusions in Escherichia coli, Arch. Microbiol., 156:444-448 (1991).
Guzzo et al., Transcription of the Escherichia coli fliC Gene Is Regulated by Metal Ions, Appl. Environ. Microbiol., 57(8):2255-2259 (1991).
Guzzo and DuBow, A luxAB transcriptional fusion to the cryptic celF gene of Escherichia coli displays increased luminescence in the presence of nickel, Mol. Gen. Genet., 242:455-460 (1994).
Kragelund et al., Isolation of lux reporter gene fusions in Pseudomonas fluorescens DF57 inducible by nitrogen or phosphorus starvation, FEMS Microbiol. Ecology, 17:95-106 (1995).
Waterfield et al. The isolation of lactococcal promoters and their use in investigating bacterial luciferase synthesis in Lactococcus lactis, Gene, 165:9-15 (1995).
Shen and Keen. Characterization of the promoter of avirulence gene D form Pseudomonas Syringae pv. tomato. J. Bact. vol. 175(18):5916-5924, Sep. 1993.
Barnes. Variable patterns of expression if luciferase in transgenic tobacco leaves. PNAS (USA). vol. 87:9183-9197, Dec. 1990.
Cebolla et. al.. Expression vectors for the use of eukaryotic luciferases as bacterial markers with different colores of luminescence. Appl. and Environ. Microbiol. vol. 61(2):660-668, Feb. 1995.
Weltring. A method for easy isolation of promoter fragments from promoter-probe libraries of filamentous fungi. Curr. Genet. 28:190-196, Aug. 17, 1995.
LaRossa Robert Alan
Van Dyk Tina Kangas
Brusca John S.
E. I. du Pont de Namours and Company
Sandals William
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