Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1998-01-22
1999-11-30
Carlson, Karen Cochrane
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 435325, 4352523, 536 231, 530350, C12P 2106, C12N 1500, C07H 1700, C07K 1400
Patent
active
059940999
ABSTRACT:
Cloned DNA encoding spider flagelliform silk proteins described. The translated amino acid sequence of the cloned cDNA shows that the flagelliform silk protein is composed largely of repeated motifs. The dominant motif of this protein is Gly-Pro-Gly-Gly-X, which can appear up to 63 times in tandem arrays. This motif likely forms Pro.sup.2 -Gly.sup.3 type II .beta.-turns. The resulting series of concatenated .beta.-turns are thought to form a helix (.beta.-spiral). The present inventors propose that this spring-like .beta.-spiral is the basis of the elasticity of silk.
REFERENCES:
C. Hayashi et al., Evidence from Flagelliform Silk cDNA for the Structural Basis of Elasticity and Modular Nature of Spider Silks, J. Mol. Biol., vol. 275, pp. 773-784 (1998).
M. Xu et al., Structure of a Protein Superfiber: Spider Dragline Silk, Proc. Natl. Acad. Sci. USA, vol. 87, pp. 7120-7124 (1990).
M. B. Hinman et al., Isolation of a Clone Encoding a Second Dragline Silk Fibrion, J. Biol. Chem., vol. 267, No. 27, pp. 19320-19324 (1992).
A. Simmons et al., Molecular Orientation and Two-Component Nature of the Crystalline Fraction of Spider Dragline Silk, Science, vol. 271, pp. 84-87 (1996).
J. Kummerlen et al., Local Structure in Spider Dragline Silk Investigated by Two-Dimensional Spin-Diffusion Nuclear Magnetic Resonance, Macromolecules, vol. 29, pp. 2920-2928 (1996).
S. L. Stauffer et al., Comparison of Physical Properties of Three Silks from Nephilia Clavipes and aAraneus Gemmoides, The Journal of Arachnology, vol. 22, pp. 5-11 (1994).
K. Mita et al., Highly Repetitive Structure and its Organization of the Silk Fibroin Gene, J. Mol. Evol., vol. 38, pp. 583-592 (1994).
F. Vollrath et al., Modulation of the Mechanical Properties of Spider Silk by Coating with Water, Nature, vol. 340 pp. 305-307, (1989).
T. Kohler et al., Thread Biomechanics in the Two Orb-Weaving Spiders Araneus Diadematus . . . and Uloborus Walckenaerius . . . , Journal of Experimental Zoology, vol. 271, pp. 1-17 (1995).
Paul A. Guerette et al., Silk Properties Determined by Gland-Specific Expression of a Spider Fibroin Gene Family, Sciencevol. 272, pp. 112-115 (1996).
J. P. O'Brien et al., Design, Synthesis, and Fabrication of a Novel Self-Assembling Fibrillar Protein, Am. Chem. Soc., ACS Symposium Series 554, pp. 105-117 (1994).
Hayashi Cheryl Y.
Lewis Randolph V.
Carlson Karen Cochrane
The University of Wyoming
LandOfFree
Extremely elastic spider silk protein and DNA coding therefor does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Extremely elastic spider silk protein and DNA coding therefor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Extremely elastic spider silk protein and DNA coding therefor will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1670439