Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Patent
1996-10-08
1999-11-16
Tsang, Cecilia
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
514828, 514855, 514859, 514863, 514886, 514887, 530400, 530412, 530414, 530415, 530417, 530418, 530427, A61K 3800, C07K 100, A21J 100
Patent
active
059858397
ABSTRACT:
The present invention relates to shark cartilage extracts and to a method of producing the same, these extracts having anti-angiogenic properties (reduction of the area of blood vessels observed in vivo on experimentally induced tumors), tumor regressive activity in vivo as well as demonstrating a direct inhibitory effect on tumor cell lines. This process does not involve any denaturing solvent or product and does not involve the use of any enzymes. It consists of obtaining a blend of whole cartilage in an aqueous solution of neutral pH, preferably pure water, this blend being centrifuged and the pellet and supernatant kept for further processing. The pellet is lyophilized and tested for anti-tumor and anti-angiogenic activities in vivo and in vitro, with or without supernatant. The supernatant has been shown to have anti-angiogenic and tumor regressive activities in vivo. The composition of the supernatant has then been investigated by different ways. Fractionation of this supernatant led to the characterization of some of its active components. The fractions were tested for their direct in vitro activity on cancer cell lines. Therefore, it is assumed that the non-fractionated supernatant has such an in vitro activity. Lyophilization substantially destroys the activity of these liquid fractions while no such abolition is observed in the solid extract.
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Brazeau Paul
Dupont Eric
Juneau Christian
Les Laboratories Aeterna Inc.
Matos Rick
Mohamed Abdel A.
Tsang Cecilia
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