Extraction, separation and recovery of diterpene glycosides from

Details Extraction, separation and recovery of diterpene glycosides from Extraction, separation and recovery of diterpene glycosides from

1981-05-21

1982-11-30

Brown, Johnnie R.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536127, C07H 108

Patent

active

043616976

ABSTRACT:
A process for recovering diterpene glycosides from the Stevia rebaudiana plant includes the steps of sequentially extracting plant material with a first solvent of intermediate polarity to extract plant substances which tend to interfere with a liquid chromatographic separation of the glycosides, and then with a second solvent of high polarity to extract glycosides, and chromatographically separating the extracted glycosides by introducing them onto a liquid chromatography column having a packing of an oxygen-containing organic stationary phase covalently bonded through a silicon atom to an inorganic support, eluting them with a solvent of polarity higher than that of the first solvent but lower than that of the second solvent, and collecting individually eluate fractions rich in respective glycosides.

REFERENCES:
patent: 3723410 (1973-03-01), Persinos
patent: 4082858 (1978-04-01), Morita et al.
patent: 4109075 (1978-08-01), Deaton
patent: 4171430 (1979-10-01), Matsushita et al.
patent: 4256875 (1981-03-01), Gabriel et al.
Y. Hashimoto et al., Shoyakugaku Zasshi 32:209, [1978].
Y. Hashimoto et al., J. Chromatography, 161:403, [1978].
M. S. Ahmed et al., J. Chromatography, 192:387, [1980].

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