Extraction of human interleukin-4- from bacteria

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.

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530412, 530418, 530419, 530420, 530422, 530423, 530424, 530427, 530825, 435 695, 435 6952, 435 711, C07K 312

Patent

active

049580078

ABSTRACT:
A method is provided for extracting human interleukin-4 (IL-4) from IL-4 expressing bacterial cells. The method comprises treating the bacterial cells with a deactivating agent, disrupting the cell membrane and recovering the IL-4.

REFERENCES:
patent: 4364863 (1982-12-01), Leibowitz
patent: 4405601 (1983-09-01), McEntire et al.
patent: 4675387 (1987-06-01), Karant
Marston, Biochem. J., 240, 1986, pp. 1-12.
Menge et al., Develop. Biol. Std., 66, 1987, pp. 391-401.
European Journal of Biochemistry, vol. 173, No. 1, Apr. 1988, pp. 109-114.

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