Extraction of heterologous insoluble proteins from bacteria

Details Extraction of heterologous insoluble proteins from bacteria Extraction of heterologous insoluble proteins from bacteria

1994-06-22

1995-08-01

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

435 691, 435 6952, 435 711, 4351723, 435948, 530351, 530412, 530420, 530427, 530825, C12P 2100, C12N 1500

Patent

active

054379865

ABSTRACT:
An insoluble mammalian protein is extracted from transformed bacteria expressing the mammalian protein while avoiding irreversible insolubilization of bacterial host proteins by homogenizing the fermentation broth, centrifuging the homogenized broth and removing the supernatant liquid for the inclusion body containing pellet. In another embodiment, the pH of the homogenized broth is adjusted to 2.0 prior to centrifugation. The acidified broth is then centrifuged, and the pellet is resuspended in buffer, homogenized again, and the inclusion body is isolated by centrifugation.

REFERENCES:
patent: 4512922 (1985-04-01), Jones et al.
patent: 4518526 (1985-05-01), Olsen
patent: 4656255 (1987-04-01), Seely
patent: 4748234 (1988-05-01), Dorin et al.
patent: 4801536 (1989-01-01), Stahl et al.
patent: 4958007 (1990-09-01), Alroy et al.
patent: 5248769 (1993-09-01), Dorin

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