Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1994-07-06
1997-01-21
Wilson, James O.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 2541, 564281, C07H 2100
Patent
active
055960928
ABSTRACT:
A process is disclosed for extracting genomic DNA from a biological sample containing a cell source comprising lysing the biological sample with a first cationic detergent in a solution of high salt concentration greater than 0.5M, under heating at 40.degree. to 80.degree. C., so as to solubilize nucleic acids and proteins present in the cell source. The lysed solution is then extracted with an organic solvent by leaving the DNA in the aqueous phase and precipitating any protein present in the aqueous phase. The DNA is then precipitated from the aqueous solution by diluting it at a salt concentration of below 0.5M and adding thereto a second cationic detergent.
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Honig et al., Research in Molecular Biology 3, "Desoxyribonuclein saute-Isolierung", 1974.
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Anal. Biochemistry 180 (1989) pp. 276-278 M. B. Johns et al.
Josif Albert
Modiano Guido
Talent S.r.l.
Wilson James O.
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