Extraction of DNA by boiling cells in an alkaline phenol/guanidi

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

536 231, C12Q 168

Patent

active

059586931

ABSTRACT:
Nucleic acid probes and primers are described for detecting fungi that cause disease in humans and animals, as well as spoilage of food and beverages. These probes can detect rRNA, rDNA or polymerase chain reaction products from a majority of fungi in clinical, environmental or food samples. Nucleic acid hybridization assay probes specific for Acremonium sp., Aspergillus clavatus, Aspergillus flavus, Aspergillus fumigatus, Aspergillus glaucus, Aspergillus nidulans, Aspergillus niger, Aspergillus ochraceus, Aspergillus terreus, Aspergillus unguis, Aspergillus ustus, Beauveria sp., Bipolaris sp., Blastoschizomyces sp., Blastomyces dermatitidis, Candida albicans, Candida glabrata, Candida guilliermondii, Candida kefyr, Candida krusei, Candida lusitaniae, Candida parapsilosis, Candida tropicalis, Chrysosporium sp., Cladosporium sp., Coccidioides immitis, Cryptococcus neoformans var gattii serotype B, Cryptococcus neoformans serotype A, Cryptococcus laurentii, Cryptococcus terreus, Curvularia sp., Fusarium sp., Filobasidium capsuligenum, Filobasidiella (Cryptococcus) neoformans var bacillispora serotype C, Filobasidiella (Cryptococcus) neoformans var neoformans serotype D, Filobasidium uniguttulatum, Geotrichum sp., Histoplasma capsulatum, Malbranchea sp., Mucor sp., Paecilomyces sp., Penicillium species, Pseudallescheria boydii, Rhizopus sp., Sporothrix schenkii, Scopulariopsis brevicaulis, Scopulariopsis brumpti, Saccharomyces cerevisiae, and Trichosporon beigelii are also described.

REFERENCES:
patent: 4851330 (1989-07-01), Kohne
patent: 5288611 (1994-02-01), Kohne
patent: 5292874 (1994-03-01), Milliman
patent: 5324632 (1994-06-01), Weisburg et al.
patent: 5352579 (1994-10-01), Milliman
patent: 5364759 (1994-11-01), Caskey et al.
patent: 5407814 (1995-04-01), Pearson et al.
patent: 5501951 (1996-03-01), Milliman
patent: 5503980 (1996-04-01), Cantor
patent: 5525464 (1996-06-01), Drmanac et al.
patent: 5580971 (1996-12-01), Mitsuhashi
patent: 5593841 (1997-01-01), Hogan et al.
G. S. Sandhu et al., "Molecular Probes for Diagnosis of Fungal Infections," Journal of Clinical Microbiology, vol. 33, No. 11, pp. 2913-2919, Nov. 1995.
P. Chomczynski and N. Sacchi, "Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction," Analytical Biochemistry, vol. 162, No. 1, pp. 156-159, Apr. 1987.
Daniel Todd et al., "Detection and Differentiation of Chicken Anemia Virus Isolates by Using the Polymerase Chain Reaction," Journal of Clinical Microbiology, vol. 30, No. 7, pp. 1661-1666, Jul. 1992.
ATCC Catalogue of Filamentous FUNGI, 18th Edition, pp. 50, 112-113 (1991).
Carr, L. G. et al., "Organization of the 5.8S, 16-18S and 23-28S ribosomal RNA genes of Cephalosporium-acremonium", Current Genetics, vol. 12, pp. 209-214 (1987).
Chomczynski and Sacchi, Analytical Biochemistry, vol. 162, pp. 156-159 (1987).
Holmes et al., Journal of Clinical Microbiology, vol. 32, pp. 228-231 (1994).
Hopfer et al., Journal of Medical and Veterinary Mycology, vol. 31, pp. 65-75 (1993).
Kwon-Chung and Bennett, Medical Mycology, Lee and Febiger, 1992.
Leclerc et al., Journal of Medical and Veterinary Mycology, vol. 32, pp. 331-341 (1994).
Lott et al., Yeast, vol. 9, pp. 1199-1206 (1993).
Maiwald et al., Journal of Medical and Veterinary Mycology, vol. 32, pp. 115-122 (1994).
Makimura et al., Journal of Medical Microbiology, vol. 40, pp. 358-364 (1994).
Mitchell et al., Journal of Clinical Microbiology, vol. 32, pp. 253-255 (1994).
Moukhamedov R., et al., "Use of Polymerase Chain Reaction-Amplified Ribosomal Intergenic Sequences for the Diagnosis of Verticillium tricorpus", Phytopathology, vol. 84, pp. 256-259 (1994).
Nakamura et al, Rinsho Byuri -- Japanese Jour. of Clinical Pathology, vol. 42, pp. 676-681 (1994).
Neuveglise C., et al., "Identification of group-I introns in the 28s rDNA of the entomopathogenic fungus Beauveria brongniartii", Current Genetics, vol. 27, pp. 38-45 (1994).
Sandhu, G. S., et al., "Molecular Probes for Diagnosis of Fungal Infections", Journal of Clinical Microbiology, vol. 33, pp. 2913-2919 (1995).
Sarosi and Davies, eds., Fungal Diseases of the Lung, Raven Press, 1993.
Spreadbury et al., Journal of Clinical Microbiology, vol. 31, pp. 615-621 (1993).
Stockman et al., Journal of Clinical Microbiology, vol. 31, pp. 845-850 (1993).
Vilgalys, R. et al., "Rapid Genetic Identification and Mapping of Enzymatically Amplified Ribosomal DNA from Several Cryptococcus Species", Journal of Bacteriology, vol. 172, No. 8, pp. 4238-4246 (1990).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Extraction of DNA by boiling cells in an alkaline phenol/guanidi does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Extraction of DNA by boiling cells in an alkaline phenol/guanidi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Extraction of DNA by boiling cells in an alkaline phenol/guanidi will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-701968

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.