Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1995-03-07
1996-04-02
Wax, Robert A.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
530380, 530412, C07K 114, C07K 14745, A61K 3512
Patent
active
055041930
ABSTRACT:
The Prothrombin Time (PT) is used as a screening test for blood coagulation factor deficiencies and for monitoring oral anti-coagulant therapy using coumadin. Thromboplastin reagents activate the extrinsic pathway of coagulation and are the basis for the Prothrombin Time (PT) test. This invention describes the use of barium sulfate and chaotropic agents, and nonionic detergents, for the extraction of sensitive thromboplastin reagents from tissue. Extraction with sodium thiocyanate alone also greatly enhances thromboplastin sensitivity. This invention should be useful for all thromboplastins and will improve their sensitivity for all PT-based tests and specific assays.
REFERENCES:
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Hatefi, Y. et al., 1974, Methods in Enzymology, 31:770-790.
Van Renswoude, J., et al., 1984, Methods in Enzymology 104:329-.
Sueshi, K., et al., 1982, Biochimica et Biophysica Acta 717(2):327-336.
Ohnishi, T., et al., 1985, The Journal of Biological Chemistry 260(5):2782-2788.
Hawkins Pamela L. H.
Maynard James R.
Moore William W.
Tymeson Cynthia G.
Wax Robert A.
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