Extraction methods for preparing thromboplastin reagents

Details Extraction methods for preparing thromboplastin reagents Extraction methods for preparing thromboplastin reagents

1993-05-25

1995-06-20

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

530381, 530384, 436 69, C12Q 156, C07K 14745, G01N 3386

Patent

active

054260310

ABSTRACT:
The Prothrombin Time (PT) is used as a screening test for blood coagulation factor deficiencies and for monitoring oral anti-coagulant therapy using coumadin. Thromboplastin reagents activate the extrinsic pathway of coagulation and are the basis for the Prothrombin Time (PT) test. This invention describes the use of barium sulfate and chaotropic agents, and nonionic detergents, for the extraction of sensitive thromboplastin reagents from tissue. Extraction with sodium thiocyanate alone also greatly enhances thromboplastin sensitivity. This invention should be useful for all thromboplastins and will improve their sensitivity for all PT-based tests and specific assays.

REFERENCES:
patent: 5017556, O'Brien et al.
B. A. Bradlow et al. "A Human Brain Thromboplastin Standard for Distribution in South Africa" S. Africa Med. J. 50(50) 1989-1992 (1976).
K. J. Stevenson et al, "The British Comparative Thromboplastin: . . . " Br. J. Haematology 44(3) 495-501 (1980).
A. J. Quick "Thromboplastin as a Reagent" Thrombosis et Diathesis Haemorrhagica 23(3) 585-592 (1920).
H. Starr et al, "Prothrombin Times: An Evaluation of Four Thromboplastins and Four Machines" Pathology 12(4) 567-574 (1980).
Guha et al. (1986) Proc. Natl. Acad. Sci. U.S.A. 83:299-302.
Bach et al. (1981) J. Biol. Chem. 256(16):8324-8331.
Bjorklid et al. (1977) Biochem. J. 165:89-96.

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