Extraction methods and assays for feed enzymes

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

Reexamination Certificate

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C436S178000

Reexamination Certificate

active

07629139

ABSTRACT:
This invention relates to methods of the detection of enzymatically active enzymes in feed added in the pre-pelleting stage. Further, this invention relates to the field of detecting thermotolerant enzymes added to feed in the pre-pelleting stage. In particular, the invention relates to the detection of a phytase enzyme, in particular anE. coliphytase or anE. coliderived phytase in feed, such as Quantum™ Phytase.

REFERENCES:
patent: 5720971 (1998-02-01), Beauchemin et al.
patent: 2001/0055788 (2001-12-01), Short et al.
patent: 2005/0009116 (2005-01-01), Yarnell et al.
Augspurger et al. Efficacy of anE. coliPhytase Expressed in Yeast for Releasing Phytate-Bound Phosphorus in Yound Chicks and Pigs; Journal of Animal Science, vol. 81 (2003) pp. 474-483.
Barrientos et al. Specificity of Hydrolysis of Phytic Acid by Alkaline Phytase From Lily Pollen; Plant Physiology, vol. 106 (1994) pp. 1489-1495.
Sigma-Aldritch Bioultra Biological Buffers, 2008 downloaded Dec. 18, 2008 from: http://www.sigmaaldrich.com/life-science/metabolomics/bioultra-reagents/biological-buffers.html.
Garrett et al., “Enhancing the Thermal Tolerance and Gastric Performance of a Microbial Phytase for Use as a Phosphate-Mobilizing Monogastric-Feed Supplement”, Applied and Environmental Microbiology, May 2004, p. 3041-3046.
Engelen et al., Determination of Phytase Activity in Feed by a Colorimetric Enzymatic Method: Collaborative Interlaboratory Study, Journal of AOAC International, vol. 84. No. 3, 2001.

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