Extraction composition, test kit and their use to extract or det

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435975, 435961, 435259, 252117, 252156, 252548, G01N 33571

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active

050810103

ABSTRACT:
An extraction composition has been found useful for extracting antigen from herpes simplex virus. This composition has a pH of from about 8.5 to about 12, and comprises an alcoholamine or salt thereof, a nonionic surfactant comprised of a condensation product of an alkylphenol and ethylene oxide, cholic acid or a salt or derivative thereof and an anionic surfactant. Extraction of antigen is accomplished by contacting the extraction composition with a specimen suspected of containing herpes organisms under suitable conditions. Extracted antigen can be determined by forming an immunological complex with antibodies thereto and by detecting that complex. The extraction composition can be supplied as part of a diagnostic test kit.

REFERENCES:
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patent: 4452734 (1984-06-01), Larson et al.
patent: 4497899 (1985-02-01), Armstrong et al.
patent: 4572896 (1986-02-01), Hampar et al.
patent: 4661349 (1987-04-01), Kino et al.
patent: 4695537 (1987-09-01), Dorsett
patent: 4847199 (1989-07-01), Snyder et al.
The Merck Index 10th ed., M. Windholz, editor 1983 pp. 541 and 1381.
Fucillo et al., "Rapid Viral Diagnosis" Manual of Clinical Laboratory Immunology 1986 pp. 489-496.
Zweig et al., J. Virology 47(1), pp. 185-192 (1983).

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