Extraction and purification of phosphodiesterase 1

Details Extraction and purification of phosphodiesterase 1 Extraction and purification of phosphodiesterase 1

2006-01-03

2006-01-03

Lankford, Jr., Leon B. (Department: 1651)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S195000, C435S196000, C435S183000, C435S814000, C800S320000

Reexamination Certificate

active

06982158

ABSTRACT:
A process for purifying a phosphodiesterase 1 (PDE-1) from a cell including heating an extract of a cell formed from a solution including at least one divalent cation, to increase the specific activity of PDE-1 in the extract.

REFERENCES:
patent: 3516907 (1970-06-01), Holle et al.
patent: 5879916 (1999-03-01), De-Eknamkul et al.
Harvey et al, 1967, Biochemistry, vol. 6, No. 12, pp. 3689-3694.
Hsin et al, 1998, Blood, vol. 92, No. 9, pp 3268-3276.
IUBMB Enzyme Nomenclature, “EC 3.1.4.1” accessed Aug. 12, 2004 www.chem.qmul.ac.uk.iumbm/enzyme/EC3/1/4/1.html.
Chae, H.J., et al., “Utilization of Brewer's Yeast Cells for the Production of Food-Grade Yeast Extract. Part 1: Effects of different enzymatic treatments on solid and protein recovery and flavour characteristics”, Bioresource Technology 76(3):253-258, 2000.
Kozma, P., et al., “Purification and Properties of a Phosphodiesterase and Nucleotide Pyrophosphatase From Root Callus Tissues of Vine Shoots”, Acta Agronomica Academiae Scientiarum Hungaricae 28(3-4):281-294, 1979.
La Marra-Phillips, A., “A Study of Phosphodiesterase in the fission yeast Schizosaccharomyces Pombe”, UMI, Order No. DA9818617, From: Diss Abstr. Int., B 58(12):6377, 1998.
Nakabayashi, T., et al., “Phosphodiesterase I in Cultured Cells ofMentha Arvensis”, Phytochemistry 39(5):1013-1016, 1995.
Sander, M., et al., “Partial Purification of Pdel fromSaccharomyces cerevisiae: Enzymatic Redundancy for the Repair of 3′-Terminal DNA Lesions and Abasic Sites of Yeast”, Biochemistry 36(20):6100-6106, 1997.
Ueda, N., et al., “Marked Activation of theN-acylphosphatidylethanolamine-hydrolyzing phosphodiesterase by divalent cations”, Biochimica et Biophysica Acta 1532(1-2):121-127, 2001.
Vogel, A., et al.,ElaCEncodes a Novel Binuclear Zinc Phosphodiesterase, Journal of Biological Chemistry 277(32), 29078-29085, 2002.
Washburn, K.B., et al., “Comparison of Mechanical Agitation and Calcium Shock Methods for Preparation of a Membrane Fraction Enriched in Olfatory Cilia”, Chemical Senses 27(7):635-642, Sep. 2002.
Wold, W.S.M., et al., “Demonstration inAspergillus nigerof adenyl cyclase, a cyclic adenosine 3′, 5′-monophosphate-binding protein, and studies on intracellular and extracellular phosphodiesterases” Canadian Journal of Microbiology, 20(11):1567-1576, 1974.
Yu, J., et al., “Identification and Characterisation of a Human Calmodulin-Stimulated Phosphodiesterase PDE1B1”, Cellular Signalling 9(7):519-529, Nov. 1997.

Affiliated with

Also associated with

Rating

Extraction and purification of phosphodiesterase 1 does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Extraction and purification of phosphodiesterase 1, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Extraction and purification of phosphodiesterase 1 will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3561548