Extraction and purification of biologically active lymphokines

Details Extraction and purification of biologically active lymphokines Extraction and purification of biologically active lymphokines

1981-05-21

1983-09-20

Roberts, Elbert L.

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

A61K 3512, A01N 6302

Patent

active

044056010

ABSTRACT:
A biologically active lymphokine fraction which exhibits macrophage activation activity and lymphotoxin activity is prepared from large scale supernatant culture fluids of human lymphoblastoid cultured cell lines grown in the presence of human serum to minimize antigenicity. Growth of lymphoblastoid cells from human lymphoblastoid cultured cell lines is effected and the supernatant culture fluids are harvested, concentrated and clarified. The supernatant culture fluids are then extracted with trichloroacetic acid, guanidine hydrochloride, sodium dodecyl sulfate or perchloric acid as the extracting solvent followed by removal of the solvent. Further purification of the lymphokine fraction can be achieved by treatment with guanidine hydrochloride or sodium dodecyl sulfate as a dissociating solvent and by subjecting the lymphokine fraction to gel filtration, sodium dodecyl sulfate gel electrophoresis and/or high pressure liquid chromatography.

REFERENCES:
"Nature", vol. 224, Oct. 4, 1969, pp. 38-42, Article by Dumonde et al.

Affiliated with

Also associated with

Rating

Extraction and purification of biologically active lymphokines does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Extraction and purification of biologically active lymphokines, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Extraction and purification of biologically active lymphokines will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-587740