Liquid purification or separation – Processes – Liquid/liquid solvent or colloidal extraction or diffusing...
Patent
1988-06-16
1989-05-23
Therkorn, Ernest G.
Liquid purification or separation
Processes
Liquid/liquid solvent or colloidal extraction or diffusing...
210656, 424 9463, 424 9464, 424 95, 435212, 435219, 435226, 435815, 514 21, 514822, 530324, 530344, 530350, 530413, 530416, 530417, 530855, B01D 1508
Patent
active
048328499
ABSTRACT:
Applicant has isolated a Factor X.sub.a inhibiting substance from the salivary gland extract of the leech H.ghilianii. The extract is subjected to separation using both DEAE-cellulose and heparin-agarose chromatography resins eluting with an increasing salt gradient. The extract is subjected to affinity chromatography using Factor X.sub.a bound to an Affi-Gel-15 resin eluting with HEPES containing benzamidine. Reverse phase chromatography yields several purified peptides or proteins having Factor X.sub.a inhibiting activity. The properties and clinical compositions of these FX.sub.a inhibitors are described.
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Merrell Dow Pharmaceuticals Inc.
Nesbitt Stephen L.
Therkorn Ernest G.
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