Extraction and purification method of active constituents...

Drug – bio-affecting and body treating compositions – Plant material or plant extract of undetermined constitution... – Containing or obtained from a stem – stalk – branch – or twig

Reexamination Certificate

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Reexamination Certificate

active

10527139

ABSTRACT:
Disclosed is a method for extracting and purifying active constituents from honeysuckle (Lonicera japonicaThunb.) and its use. More particularly, this invention relates to an extraction and purification method of active constituents including sweroside from for honeysuckle stem (stem of honeysuckle where leaves are removed) by removing tannins, hardly soluble flavonoids, saponins, and the like. Thus obtained active constituents have better anti-inflammatory and analgesic effect, are safer and more stable than the conventional active constituents obtained from honeysuckle flower or honeysuckle leaves, and include sweroside which is an effective active ingredient of anti-inflammatory and analgesic drug.

REFERENCES:
patent: 1616403 (2005-05-01), None
patent: 1998-0163813 (1998-12-01), None
patent: 2001-0267060 (2001-03-01), None
Prasad et al., A new secoiridoid glycoside fromLonicera augustifolia, Fitoterapia vol. 71, 2002, pp. 420-424.
Machida et al., Studies on the Constituents of Lonicera Species. XVII. New Iridoid Glycosides of the Stems and Leaves ofLonicera japonicaThunb, Chem.Pharm. Bull. vol. 50(8), 2002, pp. 1041-1044.
Lee, Song Jin et al., “Anti-inflammatory Activity of the Major Constituents ofLonicera japonica” Arch. Pharm. Res.1995;18(2): pp. 133-135.
Lee, S.J. et al., “Antiinflammatory Activity ofLonicera japonica” Phytotherapy Research. vol. 12, 1998, pp. 445-447.
Recio, MC et al., “Structural considerations on the iridoids as anti-inflammatory agents”,Planta Med.Jun. 1994; 60(3): Abstract Only of pp. 232-234.
Kwak, Wie Jong et al., “Loniceroside C, an Antiinflammatory Saponin fromLonicera japonica” Chem. Pharm.Bull.vol. 51(No. 3) Mar. 2003; pp. 333-335.
Moon, Tae Chul et al., “Anti-inflammatory Activity of the Flavonoid Components ofLonicera japonica” J. Pharm. Soc. Korea1999, vol. 43 (No. 1): pp. 117-123.
Tae, J. et al., “Anti-inflammatory effect ofLonicera japonicain proteinase-activated receptor 2-mediated paw edema.”Clin. Chim. ActaApr. 2003 (1-2): Abstract only of pp. 165-171.

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