Extracting protease-inhibitor from animal tissue containing same

Details Extracting protease-inhibitor from animal tissue containing same Extracting protease-inhibitor from animal tissue containing same

1978-06-19

1980-08-05

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Treating animal or plant material or micro-organism

C07G 700

Patent

active

042162939

ABSTRACT:
Protease inhibitor is extracted from fresh or frozen organs of slaughtered animals by an enzymolysis operation which excludes any possibility of interference by azymic autolysis, the enzymolysis being stopped after a time not exceeding 4 hours, whereafter a lysate aqueous solution is obtained by filtration and a quaternary ammonium base is added to the lysate solution to precipitate insolubles, the filtrate being the fraction which contains the expected protease inhibitor.

REFERENCES:
patent: 3451996 (1969-06-01), Sumyk et al.
Methods in Enzymology, vol. 19, pp. 848-850 (1971).
Chemical Abstracts, 1973, vol. 79, 51999W (Abstract of U.S.S.R. Inv. Certif. 377,159, Apr. 1973).

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