Extract solution for cell-free protein synthesis, method for...

Details Extract solution for cell-free protein synthesis, method for... Extract solution for cell-free protein synthesis, method for...

2007-03-13

2007-03-13

Lilling, Herbert J. (Department: 1651)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

C435S069100, C435S183000, C435S348000

Reexamination Certificate

active

10315108

ABSTRACT:
The present invention provides a novel extract solution for cell-free protein synthesis, which contains at least an extract derived from aBombyx moriL. tissue and an exogenous mRNA, which is easy to prepare and which is capable of realizing cell-free protein synthesis that can synthesize glycoprotein, a cell-free protein synthesis method using it, and a method for preparing an extract solution for cell-free protein synthesis, which includes adding an exogenous mRNA to an extract derived from aBombyx moriL. tissue.

REFERENCES:
patent: 5646014 (1997-07-01), Hara
patent: 2002/0168705 (2002-11-01), Nunokawa et al.
patent: 2000-236896 (2000-09-01), None
patent: 2000-325076 (2000-11-01), None
Manley et al., “DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract”,Proc. Natl. Acad. Sci. USA,vol. 77, No. 7, pp. 3855-3859, 1980.
Suzuki et al., “Differential Transcription of the Fibroin and Sericin-1 Genes in Cell-Free Extracts”,Develop. Growth&Differ.,vol. 32, No. 2, pp. 179-187, 1990.
Murakami et al., “Production of Biologically Active Recombinant Bovine Interferon- γ by Two Different Baculovirus Gene Expression Systems Using Insect Cells and Silkworm Larvae”,Cytokine,vol. 13, No. 1, pp. 18-24, 2001.

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