Extracellular serine protease and a Bacillus subtilis alkaline n

Details Extracellular serine protease and a Bacillus subtilis alkaline n Extracellular serine protease and a Bacillus subtilis alkaline n

1989-11-27

1996-12-17

Jacobson, Dian C.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435221, 43525231, 4353201, 536 232, 536 237, C12N 121, C12N 954, C12N 1555, C12N 1575

Patent

active

055852530

ABSTRACT:
A cloned gene (epr) encoding a novel extracellular protease, Epr, from Bacillus subtilis is described. Also described is a triple extracellular neutral, alkaline and serine protease deficient Bacillus subtilis mutant strain having deletions in the (npr), (apr) and (epr) genes encoding these proteases. The triple mutant strain was constructed by the gene conversion technique and produces about 1% of the extracellular proteolytic activity of the wild type. It is a particularly useful host for the production of heterologous proteins that are secreted into the growth medium.

REFERENCES:
patent: 4394443 (1983-07-01), Weissman et al.
Honjo, M., et al., J. Biotechnol (1986) 4:63-71.
Sloma et al. 1988. J. Bacteriol. 170, 5557-5563.
Roitsch et al. 1983. J. Bacteriol. 155, 145-152.
Hunkapiller et al. 1983. Meth. Enzymol. 91, 399-413.
Fahnestock et al 1987. Appl. Environ. Microbiol. 53, 379-384.

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