Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-10-28
2000-05-09
Carlson, Karen Cochrane
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
43525231, 4353201, C12P 2106, C12N 120, C12N 1500
Patent
active
060602740
ABSTRACT:
The present invention relates to Bacillus hosts transformed with a vector comprising a DNA sequence encoding for a cellulose binding domain (CBD) and capable of expressing said sequence, the expressed polypeptide protein consisting essentially of one or more non-catalytic domains; the cellulose binding domain having a molecular weight in the range of from 4 kD to 35 kD and being obtainable from a microorganism or from a plant, preferably from a bacterium or a fungus; the Bacillus host e.g. being one of the species Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus stearothermophilos, and Bacillus amyloliquefaciens; and a Bacillus expression vector carrying an inserted DNA sequence encoding for a cellulose binding domain; and a method for producing a cellulose binding domain polypeptide in a Bacillus host cell.
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Creighton, T.E. Proteins: Structures and Molecular Properties, 2nd edition. W.H. Freeman and Company, New York, pp. 23-28, 1993.
Hansen et al. celA from Bacillus lautus PL236 encodes a novel cellulose-binding endo-beta-1,4-glucanase. J. Bacteriol. 174(11):3522-3531, Jun. 1992.
Rapoport et al. Gene expression and using Bacillus. Current Opinion in Biotechnology 1:21-27, 1990.
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Chalfie et al., Science, vol. 263, pp. 802-805 (Feb. 11, 1994).
Bjornvad Mads Eskelund
Jorgensen Per Lina
Schulein Martin
Carlson Karen Cochrane
Esq. Reza Green
Novo Nordisk A S
Srivastava Devesh
Zelson Esq. Steve T.
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