Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1994-06-17
1996-10-29
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435220, 43525235, C12N 948, C12N 952, C12N 120
Patent
active
055695981
ABSTRACT:
An extracellular leucine aminopeptidase was isolated from the culture medium of Streptomyces therrnonitrificans which is a Zn.sup.2+ -dependent metalloenzyme which comprises an amino acid sequence Lys-Phe-Ser-Lys-Lys-Phe-Asn-Glu (SEQ ID NO: 3) at the N-terminal thereof, and amino acid sequences of Glu-Pro-Gly-Thr-Gly-Ala-Leu-Glu-Pro (SEQ ID NO: 4) and Asn-Pro-Asp-Ile-Val-Tyr (SEQ ID NO: 5) at other regions thereof. The aminopeptidase has a pH optimum of 7.5 to 9.0, a temperature optimum of 30.degree. to 50.degree. C., and an apparent molecular weight as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis under reducing conditions of 41 to 45 kDa, and as determined by gel filtration under native state conditions of 36 to 40 kDa.
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Kim Bum J.
Kim Jung H.
Kwon Soon C.
Lee Seung J.
Lee Young M.
Lucky Limited Co.
Naff David M.
Weber Jon P.
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