Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1983-08-09
1987-01-20
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4351723, 4351721, 435317, 435253, 935 22, 935 32, 935 33, 935 38, 935 47, 536 27, C07H 1512, C12P 2100, C12N 1500, C12N 100, C12N 120
Patent
active
046379800
ABSTRACT:
A bacterial product is made by transforming a temperature sensitive lysogen with a DNA molecule which codes, directly or indirectly, for the product, culturing the transformant under permissive conditions and externalizing the product by raising the temperature to induce phage encoded functions.
REFERENCES:
Maniatis et al, 1982, Molecular Cloning A Laboratory Manual, Cold Spring Harbor Laboratory, pp. 17-27.
Kleckner, 1979, (Abstract) "DNA Sequence Analysis of Tn10 Insertions: Origin and Role of 9 bp Flanking Repetions During Tn10 Translocation", Cell V 16(u) 711-20.
Rosenberg et al., Methods in Enzymology 101:123-138, (1983).
Kleckner et al., J. Mol. Biol. 116:125-159 (1977).
Remaut et al., Gene 15:81 (1981).
Sninsky et al., Gene 16:275 (1981).
Auerbach Jeffrey I.
Rosenberg Martin
Giesser Joanne M.
Lentz Edward T.
Lourie Alan D.
SmithKline Beckman Corporation
Williams Janice E.
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