Television – Special applications – Microscope
Reexamination Certificate
2011-08-30
2011-08-30
Patel, Haresh N (Department: 2447)
Television
Special applications
Microscope
C356S326000, C356S073000, C356S419000, C382S133000
Reexamination Certificate
active
08009189
ABSTRACT:
A high speed, high-resolution flow imaging system is modified to achieve extended depth of field imaging. An optical distortion element is introduced into the flow imaging system. Light from an object, such as a cell, is distorted by the distortion element, such that a point spread function (PSF) of the imaging system is invariant across an extended depth of field. The distorted light is spectrally dispersed, and the dispersed light is used to simultaneously generate a plurality of images. The images are detected, and image processing is used to enhance the detected images by compensating for the distortion, to achieve extended depth of field images of the object. The post image processing preferably involves de-convolution, and requires knowledge of the PSF of the imaging system, as modified by the optical distortion element.
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Amann et al., “Fluorescent-Oligonucleotide Probing of Whole Cells for Determinative, Phylogene
Basiji David
Bauer Richard
Frost Keith
Hall Brian
Liang Luchuan
Amnis Corporation
Anderson Ronald M.
Patel Haresh N
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