Chemistry: molecular biology and microbiology – Vector – per se
Patent
1993-05-21
1996-04-30
Walsh, Stephen G.
Chemistry: molecular biology and microbiology
Vector, per se
536 241, C12N 1563, C12N 1579
Patent
active
055124836
ABSTRACT:
Expression vector adapted for expression of cloned genes in an animal cell comprising a steroid responsive promoter, the promoter consisting essentially of a plurality of glucocorticoid response elements (GREs), a TATA box, and an initiator element containing a transcriptional initiator site located from 20 to 50 bases from the TATA box, the promoter lacking upstream elements which bind nuclear factor I, and the vector further comprising a restriction endonuclease site downstream from the promoter for insertion of DNA to be expressed from the promoter, wherein the DNA is expressed from the vector in an animal cell.
REFERENCES:
Mader et al., "A Steroid-inducible promoter for the controlled overexpression of cloned genes in eukaryotic cells", PNAS 90:5603, 1993.
Philpott et al., "The bifunctional iron-responsive element binding protein/cytosolic aconitase . . . binding and regulation", PNAS 91:7321, 1994.
Samaniego et al., "Molecular Characterization of a Second Iron-responsive Element Binding Protein, Iron Regulatory Protein 2", J. Biol. Chem., 269(49) 30904, 1994.
Strahle et al., "Synergistic action of the glucocorticoid receptor with transcription factors", The EMBO Journal, 7(11):3389-3395, 1988.
White et al., "A Simple and Sensitive High-Throughput Assay for Steroid Agonists and Antagonists", Bio/Technology 12:1003, 1994.
Buratowski et al., "Five Intermediate Complexes in Transcription Initiation by RNA Polymerase II", 56 Cell 549, 1989.
Mitchel and Tjian, "Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding Proteins", 245 Science 371, 1989.
Ptashne and Gann, "Activators and targets", 346 Nature 329, 1990.
Lewin, "Commitment and Activation at Pol II Promoters: A Tail of Protein-Protein Interactions", 61 Cell 1161, 1990.
Zenke et al., "Multiple sequence motifs are involved in SV40 enhancer function", 5 EMBO J. 387, 1986.
Jantzen et al., "Cooperativity of Glucocorticoid Response Elements Located Far Upstream of the Tyrosine Aminotransferase Gene", 49 Cell 29, 1987.
Green and Chambon, "Nuclear receptors enhance our understanding of transcription regulation", 4 Trends in Genet 309, 1988.
Evans, "The Steroid and Thyroid Hormone Receptor Superfamily", 240 Science 889, 1988.
Wahli and Martinez, "Superfamily of Steroid nuclear receptors: positive and negative regulators of gene expression", 5 FASEB J. 2243, 1991.
Gronemeyer, "Transcription Activation by Estrogen and Progesterone Receptors", 25 Ann. Rev. Genet. 89, 1991.
Montminy et al., "Regulation of cAMP-inducible genes by CREB", 13 Trends in Neurolog. Sciences 184, 1990.
Angel and Karin, "The role of Jun, Fos and the AP-1 complex in cell=proliferation and transformation", 1072 Biochim. Biophys. Acta. 129, 1991.
Lin et al., "Casein Kinase II Is a Negative Regulator of c-Jun DNA Binding and AP-1 Activity", 70 Cell 777, 1991.
Bauerle, "The inducible transcription activator NF-kB: regulation by distinct protein subunits", 1072 Biochin. Biophys. Acta. 63, 1991.
Hunter and Karin, "The Regulation of Transcription by Phosphorylation", 70 Cell 375, 1992.
Krust et al., "The chicken oestrogen receptor sequence: homology with v-verbA and the human oestrogen and glucocorticoid receptors", 5 EMBO J. 891, 1986.
Giguere et al., "Functional Domains of the Human Glucocorticoid Receptor", 46 Cell 645, 1986.
Kumar et al., "Functional Domains of the Human Estrogen Receptor", 51 Cell 941, 1987.
Kumar and Chambon, "The Estrogen Receptor Binds Tightly to its Responsive Element as a Ligand-Induced Homodimer", 55 Cell 145, 1988.
Green and Chambon, "Oestradiol induction of a glucocorticoid-responsive gene by a chimaeric receptor", 325 Nature 75, 1987.
Evans and Hollenberg, "Zinc fingers: Gilt by Association", 52 Cell 1, 1988.
Schwabe et al., "Solution structure of the DNA-binding domain of the oestrogen receptor", 348 Nature 458, 1990.
Hard et al., "Solution Structure of the Glucocorticoid Receptor DNA-Binding Domain", 249 Science 157, 1990.
Luisi et al., "Crystallographic analysis of the interaction of the glucocorticoid receptor with DNA", 352 Nature 497, 1991.
Mader et al., "Three amino acids of the oestrogen receptor are essential to its ability to distinguish an oestrogen from a glucocorticoid-responsive element", 338 Nature 271, 1989.
Umesomo and Evans, "Determinants of Target Gene Specificity for Steroid/Thyroid Hormone Receptors", 57 Cell 1139, 1989.
Danielsen et al., "Two Amino Acids within the Knuckle of the First Zinc Finger Specify DNA Response ELement Activation by the Glucocorticoid Receptor", 57 Cell 1131, 1989.
Tora et al., "The Human Estrogen Receptor Has Two Independent Nonacidic Transcriptional Activation Functions", 59 Cell 447, 1989.
Martinez et al., "The estrogen-responsive element as an inducible enhancer: DNA sequence requirements and conversion to a glucocorticoid-responsive element", 6 EMBO J. 3719, 1987.
Burch et al., "Two Functional Estrogen Response Elements Are Located Upstream of the Major Chicken Vitellogenin Gene", 8 Mol. Cell. Biol. 1123, 1988.
Ponglikitmongkol et al., "Synergistic activation of transcription by the human estrogen receptor bound to tandem responsive elements", 9 EMBO J. 2221, 1990.
Schule et al., "Cooperativity of the glucocorticoid receptor and the CACCC-box binding factor", 332 Nature 87, 1988.
Lee et al., "Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary tumour virus chimaeric plasmids", 294 Nature 259, 1984.
Ucker et al., "Mammary Tumor virus DNA Contains Sequences Required for Its Hormone-Regulated Transcription", 27 Cell 257, 1981.
Metzger et al., "The human oestrogen receptor functions in yeast", 334 Nature 31, 1988.
Payvar et al., "Sequence-Specific Binding of Glucocorticoid Receptor to MTV DNA at Sites within and Upstream of the Transcribed Region", 35 Cell 381, 1983.
Scheidereit et al., "The glucocorticoid receptor binds to defined nucleotide sequences near the promoter of mouse mammary tumour virus", 304 Nature 749, 1983.
Smale and Baltimore, "The Initiator as a Transcription Control Element", 57 Cell 103, 1989.
Mader Sylvie
White John H.
McGill University
Ulm John D.
Walsh Stephen G.
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