Expression vectors pSSA-K and pSSA-H for expression of SOD...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...

Reexamination Certificate

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C800S293000, C800S294000, C800S295000, C800S298000, C536S023200, C435S320100

Reexamination Certificate

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07897835

ABSTRACT:
The present invention relates to a recombinant expression vector, pSSA-K or pSSA-H, containing oxidative stress inducible promoter, such as sweetpotato peroxidase anionic 2 (SWEPA2), a nucleotide encoding superoxide dismutase (SOD) and a nucleotide sequence encoding ascorbate peroxidase (APX) linked to the promoter, antibiotics resistant gene, tobacco etch virus leader sequence, a polynucleotide encloding a transit peptide for chloroplast targeted expression, CaMV 35S transcription terminator, and T-DNA boarder sequence, and a method for preparing multiple stress tolerant transgenic plants using the recombinant expression vector.

REFERENCES:
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Tang et al. Selection of transgenic potato plants expressing both CuZnSOD and APX in chloroplasts with enhanced tolerance to oxidative stress. (2004) Korean J. Plant Biotechnol.; vol. 31; pp. 109-113.
Romano et al. Transgene organisation in potato after particle bombardment-mediated (co-)transformation using plasmids and gene cassettes. (2003) Transgenic Research; vol. 12; pp. 461-473.
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