Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1985-08-26
1989-10-17
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435320, 435170, 435849, 4351723, 536 27, 935 8, 935 29, 935 41, 935 42, 935 43, 935 45, 935 47, 935 61, 935 73, C12N 1500, C12N 120, C07H 1512, C12P 104
Patent
active
048747033
ABSTRACT:
The present invention provides a transcriptional and translational activating sequence derived from the lambda pL transcriptional activating sequence and the E. coli lpp translational activating sequence. The activating sequence has been cloned into recombinant DNA expression vectors into which DNA sequences encoding funtional polypeptides can be readily inserted and expressed. The activating sequence of the present invention has been shown to drive high-level expression of a bovine growth hormone derivative and a human growth hormone derivative in E. coli. Preferred expression vectors of the present invention also comprise the cI857 temperature-sensitive lambda pL repressor gene, a rop.sup.- derivative of the plasmid pBR322 replicon, and a tetracycline resistance-conferring gene.
REFERENCES:
patent: 4363877 (1982-12-01), Goodman et al.
patent: 4643969 (1987-02-01), Inouye et al.
patent: 4745069 (1988-05-01), Mayne et al.
Waldman et al. 1983 J. Biol Chemistry, (19): 11571-11575.
Cesaremi et al. 1982. Proc. Natl. Acad. Sci USA 80:3232.
Simons et al. 1984. Gene 28:55.
Kuhstoss & Rao, 1982, Abstract of Poster Presentation at the 13th Intnl. Congress of Microbiology in Boston, MA
Derynck et al., 1980, Nature 287:193.
Shimatake and Rosenberg, 1981, Nature 292:128.
Remaut et al., 1981, Gene 15:81.
Derom et al., 1982, Gene 17:45.
Simons et al., 1984, Gene 28:55.
Eli Lilly and Company
Levy Ron K.
Peet Richard C.
Whitaker Leroy
Wiseman Thomas G.
LandOfFree
Expression vectors for use in E. coli does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Expression vectors for use in E. coli, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression vectors for use in E. coli will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1743327