Expression vectors for production of foreign proteins as...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S004000, C435S006120, C435S183000, C435S252300, C435S320100, C435S252800, C530S350000, C536S023200, C536S023400, C536S023500, C536S023740

Reexamination Certificate

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06852512

ABSTRACT:
The present invention relates to novel expression vectors which can produce foreign proteins as soluble forms by using lysyl-tRNA synthetase and a process for preparing foreign proteins by using the expression vectors. Particularly, the present invention relates to the expression vectors which can provide foreign proteins as fused and soluble forms by exploiting the structure and expression pattern of lysyl-tRNA synthetase and the processes for preparing foreign proteins inE. colieffectively, which can be utilized industrially to produce active proteins in mass.

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Escalante et al., 1993, J. Biol. Chem, vol. 268(8):6014-6023.*
Brevet et al. J. Biol. Chem., 1995, vol. 270(24):14439-44.*
Commans S et al. solution structure of the anti-codon binding domain ofEscherichia colilysyl-tRNA synthetase and studies of its interaction with tRNA. 1995, J. Mol.Biol. vol. 253:100-113.
Clark R. L. et al. Roles of two lysyl-tRNA synthetases ofE.coli: Analysis of nucleotide sequences and mutant behavior. 1990, J. Bacteriol. vol. 172(6):3237-3243.
Sachdev, et al., 1998,Biochemical and Biophys. Res. Communications 244(No. 3):933-937 “Order of Fusions between Bacterial and Mammalian Proteins Can Determine Solubility in ”.

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