Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-06-09
2000-04-25
Martinell, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 694, 435476, C12P 2102
Patent
active
060542918
ABSTRACT:
An improved vector upon introduction into a suitable bacterial host containing the thermolabile repressor C.sub.I renders the host cell capable, upon increasing the temperature of the host cell to a temperature at which the repressor is destroyed, of effecting expression of a desired gene inserted into the vector and production of polypeptide encoded by the gene. The vector is a double-stranded DNA molecule which includes in 5' to 3' order the following: a DNA sequence which contains the promoter and operator P.sub.L O.sub.L from lambda bacteriophage; the N utilization site for binding antiterminator N protein produced by the host cell; a DNA sequence which contains a ribosomal binding site for rendering the mRNA of the desired gene capable of binding to ribosomes within the host cell; an ATG initiation codon or a DNA sequence which is converted into an ATG initiation codon upon insertion of the desired gene into the vector; a restriction enzyme site for inserting the desired gene into the vector in phase with the ATG initiation codon; and additionally a DNA sequence which contains an origin of replication from a bacterial plasmid capable of autonomous replication in the host cell and a DNA sequence which contains a gene associated with a selectable or identifiable trait which is manifested when the vector is present in the host cell.
REFERENCES:
patent: 4578355 (1986-03-01), Rosenberg
patent: 4925799 (1990-05-01), Rosenberg
patent: 5637495 (1997-06-01), Gorecki et al.
Rosenberg et al, Methods Enzymol. 101: 123 (1983).
R. Watson, et al., FEBS Letters, 150(1): 114-116 (1982).
Gorecki Marian
Levanon Avigdor
Oppenheim Amos
Vogel Tikva
Bio-Technology General Corp.
Martinell James
White John P.
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