Expression vectors encoding bacteriophage signal peptides

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S252330, C435S320100, C536S023530, C536S023100

Reexamination Certificate

active

10483008

ABSTRACT:
Expression vectors encoding bacteriophage signal peptides are described. The vectors may be used for the heterologous expression and secretion of polypeptides such as antibodies in bacterial host cells.

REFERENCES:
patent: 6476198 (2002-11-01), Kang
patent: WO97/01580 (1997-01-01), None
Atwell et al., Mol. Immunol., vol. 33, No. 17/18, pp. 1301-1312, 1996.
Atlan and Portalier, “Optimized extracellular production of alkaline phosphatase by lky mutants ofEscherichia coliK12,” Appl. Microbiol. Biotechnol. (1984) 19:5-12.
Forgini-Lefebvre and Portalier, “Isolation and preliminary characterization of beta-lactamase excretory mutants ofEschericia coliK-12,” FEMS Micrbiol. Lett. (1984) 21:323-328.
Glover, “DNA Cloning: A Practical Approach, vol. II: Expression Systems,” IRL Press (1995).
Glover, “DNA Cloning: A Practical Approach, vol. IV: Mammalian Systems,” IRL Press (1995).
Gray, et al., “Periplasmic production of correctly processed human growth hormone inEscherichia coli: natural and bacterial signal sequences are interchangeable,” Gene (1985) 39:247-254.
Humphreys, et al., “Human protein disulfide isomerase functionally complements a dsbA mutation and enhances the yield of pactate lyase C inEscherichia coli,” J. Biol. Chem. (1995) 270:28210-28215.
Humphreys, et al., “Coexpression of human protein disulphide isomerase (PDI) can increase the yield of an antibody Fab' fragment expressed inEscherichia coli,” FEBS Lett. (1996) 380:194-197.
Humphreys, et al., “F(ab')2 molecules made fromEscherichia coliproduced Fab' with hinge sequences conferring increased serum survival in an animal model,” J. Immunol. Methods (1998) 217:1-10.
Humphreys, et al., “High-level periplasmic expression inEscherichia coliusing a eukaryotic signal peptide: importance of codon usage at the 5′ end of the coding sequence,” Protein Express. Purif. (2000) 20:252-264.
Humphreys and Glover, “Therapeutic antibody production technologies: molecules applications, expression and purification,” Curr. Opin. Drug Discovery Devel. (2001) 4:172-185.
Humphreys, et al., “A plasmid system for optimization of Fab' production inEscherichia coli: importance of balance of heavy chain and light chain synthesis,” Protein Express. Purif. (2002) 26:309-320.
Kumagai, et al., “Expression and secretion of rice alpha-amylase bySaccharomyces cerevisiae,” Gene (1990) 94:209-216.
Markland, et al., “Design, construction and function of a multicopy display vector using fusions to the major coat protein of bacteriophage M13,” Gene (1991) 109:13-19.
Sambrook and Fritsch, “Molecular Cloning: A Laboratory Manual,” 2nd ed., Cold Spring Harbor Press, New York (1989).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Expression vectors encoding bacteriophage signal peptides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Expression vectors encoding bacteriophage signal peptides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression vectors encoding bacteriophage signal peptides will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3820449

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.