Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-05-25
1998-06-02
Degen, Nancy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
43525233, 4353201, C12P 2102, C12N 121, C12N 1563, C12N 1570
Patent
active
057598169
ABSTRACT:
An improved vector upon introduction into a suitable host containing the thermolabile repressor C.sub.I renders the host capable of effecting expression of a desired gene. The vector is a double-stranded DNA molecule which includes in 5' to 3' order the following: the promoter and operator P.sub.L O.sub.L from lambda bacteriophage; the N utilization site; a first restriction enzyme site permitting replacement of the ribosomal binding site which follows thereafter; a ribosomal binding site; an ATG initiation codon or DNA which is converted into an ATG initiation codon upon insertion of the desired gene into the vector; a second restriction enzyme site for inserting the gene in phase with the ATG codon; a T.sub.1 T.sub.2 rRNA transcription termination sequence; an origin of replication and a gene associated with a selectable or identifiable phenotypic trait manifested when the vector is present in the host. The distance between the 3' end of the P.sub.L O.sub.L promoter and operator sequence and the 5' end of the N utilization site is less than about 80 base pairs and the distance between the 3' end of the N utilization site and the 5' end of the ribosomal binding site is less than about 300 base pairs. Plasmids have been constructed from the vectors and used to produce bovine, chicken and porcine growth hormones, human apolipoprotein E and human superoxide dismutase.
REFERENCES:
patent: 4363877 (1982-12-01), Goodman et al.
patent: 4436815 (1984-03-01), Hershberger et al.
patent: 4512922 (1985-04-01), Jones et al.
patent: 4559300 (1985-12-01), Kovacevic et al.
patent: 4578355 (1986-03-01), Rosenberg
patent: 4637980 (1987-01-01), Auerbach et al.
patent: 4650761 (1987-03-01), Hershberger et al.
patent: 4670393 (1987-06-01), Seeburg
patent: 4710473 (1987-12-01), Morris
patent: 5112744 (1992-05-01), Oppenheim et al.
patent: 5126252 (1992-06-01), Oppenheim et al.
patent: 5143836 (1992-09-01), Hartman et al.
patent: 5162217 (1992-11-01), Hartman et al.
patent: 5198361 (1993-03-01), Aviv et al.
patent: 5256546 (1993-10-01), Aviv et al.
Remaut, E. et al., Gene 15: 81-93 (1981).
Derynck, R. et al., Nature 287: 193-197 (1980).
Oppenheim, A.B. et al., J. Mol. Biol. 158: 327-346 (1982).
Shimatake, H. et al., Nature 292: 128-132 (1981).
Courtney, M. et al., Proc. Natl. Acad. Sci. (USA) 81: 669-673 (1984).
Lautenberger, J.A. et al., Gene 23: 75-84 (1983).
Lautenberger, J.A. et al., Science 221: 858-860 (1983).
Shatzman, A.R. et al., 14 Miami Winter Symposium, abstract p. 98 (1982).
Amann, E. et al., Gene 25: 167-178 (1983).
Gelfand, D.H. et al., Proc. Natl. Acad. Sci. (USA) 75: 5869-5873 (1978).
Zabeau, M. et al., The EMBO Journal 10: 1217-1224 (1982).
Gentz, R. et al., Proc. Natl. Acad. Sci. USA 78(8) 4936-4940 (1981).
Spence, C.A. et al., Amer. Soc. of Animal Science, abstract (University of Missouri, Aug. 7-10, 1984).
Muesing et al., Cell 24:235-242 (1981) (Exhibit 7).
Seeburg et al., DNA 2(1):37-45 (1983) (Exhibit 8).
Souza et al., J. Exp. Zool. 232(3):465-474 (1984) (Exhibit 9).
Souza et al., J. Ce.. Biochem., abstract 1070:85 (1984) (Exhibit 10).
Proudman, Proc. Soc. Exp. Biol. Med. 175(4):417-419 (1984) (Exhibit 11).
Hedgpeth et al., Mol. Gen. Genet. 163:197-203 (1978) (Exhibit 12).
Maniatis et al., In Molecular Cloning, A Laboratory Manual, Cold Spring Harbor, pp. 411-413 and 418-419 (1982) (Exhibit 13).
DeBoer et al., DNA 2(3):231-235 (1983) (Exhibit 14).
Backman et al., Cell 13:65-71 (1978) (Exhibit 15).
Guarante et al., Cell 20:543-553 (1980) (Exhibit 16).
Guarante et al., Science 209:1428-1430 (1980) (Exhibit 17).
Rosenberg et al., Methods in Enzymology 101:123-138 (1983) (Exhibit 18).
Glaser et al., Nature 302:74-76 (1983) (Exhibit 19).
Nilsson et al., Nucleic Acids REs. 11(22):8019-8030 (1983) (Exhibit 20).
Sarmientos et al., Cell 32:1337-1346 (1983) (Exhibit 21).
Williams et al., Virology 29:555-575 (1979) (Exhibit 22).
Watson et al., Recombinant DNA, A Short Course, W.H. Freeman and Company, New York (1983) (Exhibit 23).
Bernard, H.V. et al., Gene 5: 59-76 (1979).
Derom, C. et al., Gene 17: 45-54 (1982).
Gheysen, D. et al., Gene 17: 55-63 (1982).
Eppard, P.J. et al., Proceeding of 1984 Cornell Nutrition Conference for Feed Manufacturers, pp. 5-12.
Chapon (1982) EMBO J vol. 1(3):369-374.
Warburton et al. (1983), NAR, vol. 11(17)5837-5854.
Brosius (1984), Gene, vol. 27, pp. 161-172.
Brosius et al (1981), JMB, vol. 148, pp. 107-127.
Stueber et al (1982), EMBO J., vol. 1(11), pp. 1399-1404.
ATCC Recombinant DNA materials, host notes p. 116.
Holmes et al Cell 32:1029-23, 1983.
Gorecki Marian
Levanon Avigdor
Locker-Giladi Hilla
Oppenheim Amos B.
Vogel Tikva
Bio-Technology General Corp.
Degen Nancy
White John P.
LandOfFree
Expression vectors containing .lambda.P.sub.L promoter and T.sub does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Expression vectors containing .lambda.P.sub.L promoter and T.sub, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression vectors containing .lambda.P.sub.L promoter and T.sub will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1458542