Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1998-04-15
2000-11-07
Park, Hankyel
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4241991, 4242081, 435 693, 435 9142, 4351723, C12P 2106, C12N 1564, C12N 1500, A61K 3912, A61K 3921
Patent
active
061435201
ABSTRACT:
The present invention is related to vectors and methods for increasing the expression of a desired gene product. Preferably this invention is used with genes expressing proteins that are not well tolerated by mammalian cells or where high levels of expression are necessary. In certain preferred embodiments it can be used as part of a multi-tiered expression system and with methods of intracellularly targeting a molecule.
REFERENCES:
Aran J.M. et al., "Drug-Selected Coexpression of Human Glucocerebrosidase and P-glycoprotein using a Bicistronic Vector," Proc. Natl. Acad. Sci. USA 91:3176-3180 (1994).
Morgan R. A. et al., "Retroviral Vectors Containing Putative Internal Ribosome Entry Sites: Development of a Polycistronic Gene Transfer System and Applications to Human Gene Therapy," Nucl. Acids Res. 20(6):1293-1299 (1992).
Levin R. et al., "Inhibition of Early and Late Events of the HIV-1 Replication Cycle by the Cytoplasmic Fab Intrabodies Against the Matrix Protein, p17," Molec. Med. 3(2):96-110 (1997).
Chen B.-F. et al., "Characterization of a Bicistronic Retroviral Vector Composed of the Swine Vesicular Disease Virus Internal Ribosome Entry Site," J. Virol 67(4):2142-2148 (1993).
Koo H.-M. et al., "A Spleen Necrosis Virus-Based Retroviral Vector which Expresses Two Genes from a Dicistronic MrNA," Virol. 186:675 (1992).
Adam M. A. et al., "Internal Initiation of Translation in Retroviral Vectors carrying Picornavirus 5' Nontranslated Regions," J. Virol. 65:4985-4990 (1991).
T. Akagi et al., Gene, vol. 106, 1991, pp. 255-259.
M.L. Lever et al., J. Biochem., Abstract, 1993, p. 250.
M.A. Biasolo et al., Prog. Biotechnol., vol. 9, pp. 685-688.
L. Zitvogel et al., Human Gene Therapy, vol. 5, 1994, pp. 1493-1506. (w/abstract).
M. Gossen et al., Proc.Natl.Acad.Sci. USA, vol. 89, 1992, pp. 5547-5551. (w/abstract).
I.R. Ghattas et al., Mol.Cell.Biol., vol. 11, 1991, pp. 5848-5859.
D.G. Macejak et al., Nature, vol.353, 1991, pp. 90-94.
S.K. Oh et al., Genes & Development, vol. 6, 1992, pp. 1643-1653.
J. Pelletier et al., Nature, vol. 334, 1998, pp. 320-325.
P.S. Mountford et al., TIG, vol. 11, 1995, pp. 179-184.
Y. Sugimoto et al., Human Gene Therapy, vol. 6, 1995, pp. 905-915. (w/ abstract).
Marasco Wayne A.
Parolin Maria Cristina
Richardson Jennifer
Sodroski Joseph G.
Dana-Farber Cancer Institute Inc.
Park Hankyel
LandOfFree
Expression vectors and methods of use does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Expression vectors and methods of use, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression vectors and methods of use will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1639292