Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1992-09-18
1995-01-10
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 691, 435 693, 435 698, 4351723, 435193, 4353201, 43525231, 536 234, 536 237, 935 47, 935 48, C12N 1509, C12N 1554, C12N 1562, C12N 1575
Patent
active
053806530
ABSTRACT:
The invention relates to recombinant DNA molecules and to methods for producing proteins by means of said molecules. Particularly, the present invention relates to recombinant DNA molecules which are capable of being synthesized in Bacillus strain bacteria comprising the regulation and deleted non-functional signal sequence of the a-amylase gene of B. amyloliquefaciens, or a substantial part thereof, to which sequence a structural gene of any desired homologous or heterologous protein or peptide may be joined. These recombinant DNA molecules can be used, for example, to achieve intracellular expression of any desired protein or peptide in Bacillus strain bacteria.
REFERENCES:
Kallio "Expression and Regulation of the Bacillus Amyliquefaciens alpha-Amylase Gene in Bacillus subtilis," Thesis Dissertation (1987), pp. 1-27.
Palva, I., et al., Gene 15:43-51 (1981).
Palva, I., Gene 19:81-87 (1982).
Palva, I., et al., Proc. Natl. Acad. Sci. USA 79:5582-5586 (1982).
Petterson, R. F., et al, Gene 24:15-27 (1983).
Kallio, P., et al., Journal of General Microbiology 132:677-687 (1986).
Takkinen, K., et al., Journal of Biological Chemistry 258:1007-1013 (1983).
Emr, S. D., et al., Nature 285:82-85 (1980).
Fahnestock, S. R., et al., Journal of Bacteriology 165:796-804 (1986).
Sibakov, Eur. J. Biochem. 155:577-581 (1986).
Ulmanen et al., J. Bacteriol. 162(1):176-182 (1985).
LeGuyader J.
Meyer Virginia H.
Schwartz Richard A.
The Finnish National Public Health Institute
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