Expression vector system regulated by σ 32 and...

Details Expression vector system regulated by σ 32 and... Expression vector system regulated by σ 32 and...

2006-12-21

2010-10-05

Steadman, David J (Department: 1656)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S320100, C435S069100, C536S023100, C536S024100

Reexamination Certificate

active

07807460

ABSTRACT:
This invention discloses an expression vector system comprising a promoter recognized and regulated by a heat shock sigma factor ofE. coli, especially σ32. Preferably, the promoter comprises the consensus sequence ofE. coliheat shock promoters as shown in SEQ ID NO:1. Also disclosed are methods for producing proteins using the promoter under heat shock conditions. Furthermore, the present invention discloses a method for creating a sudden temperature shift in a cell culture which has been pre-cultured to reach an optimal condition and which temperature shift will allow optimal production of a recombinant protein under the control a heat shock promoter.

REFERENCES:
Wegrzyn et al., Differential inhibition of transcription form sigma70 and sigma32 dependent promoters by rifampicin., 1998, FEBS Letters, vol. 440, pp. 172-174.
Fayet et al., Suppression of theEscherichia colidnaA46 mutation by amplification of the groES and groEL genes, Mol Gen Genet. Mar. 1986, vol. 202(3), pp. 435-445.
Wang et al., Sigma 32-Dependent Promoter Activity In Vivo: Sequence Determinants of the groE Promoter, Journal of Bacteriology, Oct. 2003, vol. 185, pp. 5800-5806.

Affiliated with

Also associated with

Rating

Expression vector system regulated by σ 32 and... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Expression vector system regulated by σ 32 and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression vector system regulated by σ 32 and... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4191182