Expression vector for Phytolacca antiviral protein

Details Expression vector for Phytolacca antiviral protein Expression vector for Phytolacca antiviral protein

1994-11-21

1997-07-15

Jagannthon, Vasu

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4352528, 4353201, 536 236, C12N 1529, C12N 120, C12N 1563

Patent

active

056482345

ABSTRACT:
The present invention provides a novel expression vector for Phytolacca antiviral protein isolated from Phytolacca americana L. and a microorganism transformed with said PAP expression vector. In accordance with the present invention, biologically active PAP can be produced in a massive manner from the microorganism transformed with the expression vector of the invention; and, therefore, the recombinant PAP can be practically applied to various fields, e.g., the molecular studies on PAP and treatment of AIDS employing immunoconjugate.

REFERENCES:
patent: 4363758 (1982-12-01), Masuhu et al.
patent: 5101025 (1992-03-01), Piatak, Jr. et al.
patent: 5348865 (1994-09-01), Moon et al.
patent: 5416202 (1995-05-01), Bernhard et al.
patent: 5484889 (1996-01-01), Lee-Hwang et al.
Isolation and Analysis of a Genomic Clone Encoding a Pokeweed Antiviral Protein, (Kataoka, J.) Plant Mol.Biol., 20:879-886 (1992).
J.D. Irvin --Purification and Partial Characterization of the Antiviral Protein from Phytolacca americana which Inhibits Eukaryotic Protein from Phytolacca americana which Inhibits Eukaryotic Protein Synthesis; Arch. Biochem. Biophys., 169, 522-528 (1975).
K.C. Halling et al. --Genomic Cloning and Characterization of a Ricin Gene from Ricinus communis; Nucleic Acid Res., 13, 8019-8033 (1985).
J. Kataoka et al. --DNA Sequence of Mirabilis Antiviral Protein (MAP), a Ribosome-inactivating Protein with an Antiviral Property, from Mirabilis jalapa L. and Its Expression in Escherichia coli; J.Biol. Chem., 266, 8426-8430 (1991).
X. Zhang et al. --Homology of Trichosanthin and Ricin A Chain; Nature, 321, 477-478 (1986).
Y.Endo et al. --The RNA N-Glycosidase Activity of Ricin A-Chain; J. Biol. Chem., 263, 8735-8739 (1988).
J.D. Irvin et al. --Purification and Properties of a Second Antiviral Protein from Phytolacca americana Which inactivates Eukaryotic Ribosomes; Arch. Biochem. Biophys., 200, 418-425 (1980).
B. Jansen et al. --Establishment of a Human t(4;11) Leukemia in Severe Combined Immunodeficient Mice and successful Treatment Using Anti-CD19 (B43)-Pokeweed Antiviral Protein Immunotoxin; Cancer Res., 52, 406-412 (1992).
Y.W. Kim et al. --Immunoconjugates That Neutralize HIV Virions Kill T cells Infected with Diverse Strains of HIV-1; J. Immunol., 144, 1257-1262 (1990).
D.E. Myers et al. --Production of a Pokeweed Antiviral Protein(PAP)-containing Immunotoxin, B43-PAP, Directed Against the CD19 Human B Lineage Lymphoid Differentiation Antigen in Highly Purified Form for Human Clinical Trails; J. Immunol. Methods, 136, 221-238 (1991).
M.P. Ready et al. --Extracellular Location of Pokeweed Antiviral Protein; Proc. Natl. Acad. Sci. USA, 83, 5053-5056 (1986).
Q. Lin et al. --Isolation and characterization of a cDNA Clone Encoding the Anti-viral Protein from Phytolacca americana; Plant Mol. Biol., 17, 609-614 (1991).
Kataoka et al, "Expression of a pokeweed antiviral protein in E. coli . . . " FEBS Lett. 320(1):31-34 (Mar. 1993).
Gheran et al, ATCC Catalog, 1992, p. 137.
Lodge et al, "Characterization and Cloning of the Pokeweed Antiviral Protein", J. Cell. Biochem., Suppl. 14E:304 (1990).

Affiliated with

Also associated with

Rating

Expression vector for Phytolacca antiviral protein does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Expression vector for Phytolacca antiviral protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression vector for Phytolacca antiviral protein will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1491730