Expression vector for insulin-like growth factor I and method of

Details Expression vector for insulin-like growth factor I and method of Expression vector for insulin-like growth factor I and method of

1993-02-01

1994-08-23

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 4351723, 4352523, 43525233, 4353201, 536 235, C12N 1500, C12N 500

Patent

active

053407253

ABSTRACT:
Two-cistronic Met-IGF-I expression vector, in which the first cistron encodes a protective peptide with a molecular weight of about 500-50,000 and the second cistron encodes IGF-I, was provided. Also provided is a process for preparing Met-IGF-I, which comprises transforming E. coli with said vector and growing the resultant transformant, followed by the lysis of the cell culture and isolation of Met-IGF-I.

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J. Biochem, vol. 101, No. 5, 1987, pp. 1281-1288; Y. Saito et al.: "Direct expression of a synthetic somatostatin C gene in Eschericha coli by use of a two-cistron system" *Whole Article*.
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