Expression vector for human TNF

Details Expression vector for human TNF Expression vector for human TNF

1989-09-05

1991-10-22

Teskin, Robin L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 4353201, 43525233, 935 11, 935 29, 935 31, 935 42, 935 56, 935 65, 536 27, C07H 1512, C12P 202, B61K 3702, C12N 120

Patent

active

050595300

ABSTRACT:
The present invention provides a vector plasmid capable of efficient tumor necrosis factor (TNF) production, a process capable of efficient TNF production in a host transformed with said plasmid and a composition containing the TNF produced by said process.
The novel plasmid of the present invention is characterized by having inserted therein a DNA fragment that has a phage-derived promoter region upstream of a structural gene for TNF and in which a DNA fragment containing an E. coli gene-derived transcription termination coding base sequence (terminator) is joined immediately downstream of a base sequence coding for the termination of translation of said structural gene.

REFERENCES:
patent: 4871663 (1989-10-01), Ohshima et al.
Platt, T. Cell vol. 24, pp. D-23, Apr. 1981, "Termination of Transcription and 180 Regulation of the Tryptophehopeonin E Coli".
Lacatena, et al., Cell vol. 37, pp. 1004-1014, Jul. 19 & 7, "Control of Initiation of pMB1 Replication: Purified Rop Protein and RNA I Affect Primer Formation in Vitro".
Nature, vol. 312, Dec. 20/27, 1984-D. Pennica et al., "Human Tumor Necrosis Factor: Precursor Structor, Expression and Homology to Lymphotoxin", pp. 724, 725.
Science, vol. 228, No. 4696, Apr. 12, 1985-A. M. Wang et al., "Molecular Cloning of the Complementary DNA For Human Tumor Necrosis Factor", pp. 149-154.

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