Expression vector for animal cell containing nuclear matrix...

Chemistry: molecular biology and microbiology – Vector – per se

Reexamination Certificate

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C536S024100

Reexamination Certificate

active

10450556

ABSTRACT:
The present invention relates to mammalian expression vectors including nuclear matrix attachment region of human interferon β, and more particularly to pPGM-1, pPGM-2 and pPGM-3 including nuclear matrix attachment region of interferon β gene. Those expression vectors confer position independent expression of the introduced foreign gene, thus increasing the frequency of colonies which efficiently express the recombinant protein.

REFERENCES:
Lucas et al. High-level production of recombinant proteins in CHO cells using a dicistronic DHFR intron expression vector. Nucleic Acids Res. May 1, 1996;24(9):1774-9.
“A glimpse of Chromosomal order”, S.M. Gasser, et al., 1987 Elsevier Science Publishers.B.V. Trends Genet 3:16-22.
“Introduction of Purified Genes Into Mammalian Cells”, R. Kucherlapati, et al, CRC Critical Reviews in Biochemistry 16:349-379, 1984.
“Germ-Line Transformation of Mice”, R. Palmiter, et al., 1986 Annual Reviews Inc. Annual Rev. Genet. 20:465-499.
“Boundary functions in the control of gene expression”, J.C. Eissenberg, et al., TIG Oct., vol. 7, No. 10 335-340, 1991.
“Matrix-attachment regions can impart position-independent regulation of a tissue-specific gene in transgenic mice”, R. MCKnight, et al., Proc. Natl. Acad. Sci. USA vol. 89, pp. 6943-6947, 1992.
“Potition-Independent Transgene Expression Mediated by Boundary Elements from the Apolipoprotein B Chromatin Domain”, M. Kalos, et al., Molecular and Cellular Biology, Jan. 1995, p. 198-207.
“Scaffold-Attached Regions from the Human Interferon B Domain Can Be Used To Enhance the Stable Expression of Genes under the Control of VArious Promoters”, D. Klehr, et al., 1991 American Chemical Society Biochem. 5:1264-1270.
“Dissection of the Ability of the Chicken Lysozyme Gene 5′ Matrix Attachment Region To Stimulate Transgene Expression and To Dampen Position Effects”, L. Phi-Van, et al., 1996 American Chemical Society Biochem. 35:10735-10742.
Kraevskii, V.A., et al, “Stable bent and low-melting DNA sequences in the region of the replication origin of chicken alpha-globin gene domain”, Mol., Biol. 26 672-678, 1992.
“Anchorage of the Chinese Hamster Dihydrofolate Reductase Gene to the Nuclear Scaffold Occurs in an Intragenic REgion”, E. Kas, et al, 1987 Academic Press Limited J Mol Biol 198:677-692.
“Yeast ARS function and nuclear matrix association coincide in a short sequence from the human HPRT Locus”, R. Sykes, et al, Mol Gen Genet (1988) 212:301-309.
A-T-rich scaffold attachment regions flank the hematopoietic serine protease genes clustered on chromosome 14q11.2., Hanson RD, Ley TJ, 81md (1992 79:610-8 Abstract Only.
“Hierarchical Binding of DNA Fragments Derived from Scaffold-Attached Regions: Correlation of Properties in Vitro and Functions in Vivo”, C. Mielke, 1990 American Chemical Society.
“Scaffold Attachment Region-Mediated Enhancement of Retroviral Vector Expression in Primary T Cells”, M. Agarwal, et al., Journal of Virology, May 1998, p. 3720-3728.
“Human Beta Interferon Scaffold Attachment Region Inhibits De Novo Methylation and Confers Long-Term, Copy Number-Dependent Expression to a Retroviral Vector”, Dang et al., Journal of Virology, Mar. 2000, p. 2671-2678.
“Effects of Scaffold Attachment Region on the Transgene Expression in Retrovirus Vector-Tranduced Primary T Cells and Macrophages”, J. Auten, et al, Human Gene Therapy May 20, 1999, 1389-1399.
“Identification of the proteins responsible for SAR DNA binding in nuclear matrix of Cucurbita pepo.”, R. Markiewecz, et al., Acta Biochem Pol. (1995) 42:171-6 Abstract Only.

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