Expression vector containing an inducible selection gene system

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4353523, 4353201, C12P 2100, C12N 121, C12N 1563

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active

058405216

ABSTRACT:
The invention relates to vectors which include an inducible selection gene and a sequence which codes for a heterologous polypeptide. In a particular example the selection gene comprises the tetA and tetR genes. The vector may also include a sequence which is capable of conferring stability on the vector, such as the cer sequence, a transcription terminator, such as gene 32 from bacteriophage T4, a ribosome binding site, and a multi-cloning site. Hosts transformed with the vector, processes for preparing these hosts, and processes for preparing polypeptides using these hosts.

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