Expression systems for amidating enzyme

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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4353201, 4352523, 536 232, C12N 510, C12N 1563, C12N 121, C07H 2104

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active

057892342

ABSTRACT:
Alpha-amidating enzyme is produced by recombinant DNA techniques recoverable in high yields and at high purity. Both eukaryotic and prokaryotic expression vectors are provided having a transcriptional promoter followed downstream by a DNA sequence which encodes amidating enzyme. The vector selected is one capable of directing the expression of polypeptides in the host selected, and preferred hosts are transected with the described vectors.

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