Expression systems

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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Details

435 691, C12N 1506, C12N 1585, C12P 2100

Patent

active

055388850

ABSTRACT:
Expression systems which comprise a mammalian host, such as erythroid cells, transformed with a vector which comprises a promoter, a DNA sequence which codes for a desired polypeptide and a dominant control region. The vectors and methods for preparing polypeptides using the expression systems are also described. In a preferred embodiment, the vector contains cDNA for the desired polypeptide and a sequence which is capable of stabilising mRNA produced from the cDNA. The expression systems are particularly efficient and are capable of secreting the desired polypeptide.

REFERENCES:
patent: 4663281 (1987-05-01), Gillies et al.
Nevins. Ann. Rev. Biochem. 1983, (52) 441-466.

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