Expression system which can be regulated

Details Expression system which can be regulated Expression system which can be regulated

1996-03-12

1998-11-03

Degen, Nancy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

4352523, 43525233, 4353201, 536 231, 536 241, C12P 2102, C07H 2104, C12N 121, C12N 1574

Patent

active

058306927

ABSTRACT:
An expression system can be regulated by acetate, pH and oxygen, which expression system includes a trans-acting regulator protein and a promoter which can be activated by this protein. Any desired structural genes are maximally expressed under the control of the expression system at an oxygen partial pressure, pO.sub.2, of 0-5% and a pH of 6.0-6.5, and in the presence of acetate at a concentration of 40-60 mM. There is also a process for preparing this expression system, and a process for using this expression system.

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