Expression system for novel pullulanase

Details Expression system for novel pullulanase Expression system for novel pullulanase

1995-06-07

1998-04-07

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

43525231, 4353201, 536 232, C12N 944, C12N 120, C12N 1500, C07H 2104

Patent

active

057363757

ABSTRACT:
The invention relates to a heat-stable pullulanase having the property of hydrolysing glucosidic bonds of the .alpha.-1,6 type in amylopectin and having an enzymatic activity in an acid medium and at a temperature of about 60.degree. C.
The invention also relates to strains of microorganisms which produce this pullulanase and processes for the preparation of this pullulanase.
The invention also relates to the uses thereof and compositions comprising the product.
The invention also relates to a DNA molecule. The invention relates to an expression vector containing this DNA molecule and to a chromosomal integration vector containing this DNA molecule.

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