Expression system comprising DNA encoding the signal peptide of

Details Expression system comprising DNA encoding the signal peptide of Expression system comprising DNA encoding the signal peptide of

1994-03-01

1996-05-07

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Vector, per se

536 234, 536 235, 536 241, C12N 1576, C12N 1512, C12N 1562

Patent

active

055145906

ABSTRACT:
A DNA signal sequence initially isolated from Streptomyces griseus encodes a signal peptide which directs the secretion, via a fused intermediate, of a protein from the cell within which the DNA signal sequence is expressed. The signal sequence is derived from genes encoding protease A and protease B of S. griseus. The DNA signal sequence encodes a thirty-eight amino acid signal peptide. A DNA construct, including the DNA signal sequence and a gene sequence encoding a protein, when transformed into a living cell by a suitable vector, results in the signal peptide correctly directing the secretion of a mature protein of desired structure, particularly from prokaryotic genera selected for their ability to display enzymatic activity of a type typified by, but not exclusive to, that of protein disulphide oxidoreductase, EC 5.3.4.1, more particularly in the genera Streptomyces, and most particularly in Streptomyces lividans 66.

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