Expression, purification and uses of a Plasmodium...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S007100, C424S191100, C530S350000

Reexamination Certificate

active

07550275

ABSTRACT:
In this application is described the expression and purification of a recombinantPlasmodium falciparum(3D7) LSA-NRC polypeptide. The method of the present invention produces a highly purified polypeptide which is useful as a vaccine and as a diagnostic reagent.

REFERENCES:
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Fidock et al., 1994. Plasmodium falciparum liver stage antigen-1 is well conserved and contains potent B and T cell determinants. J. Immunol. 153, 190-204.
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Connelly et al., 1997. T-Cell immunity to peptide epitopes of liver-stage antigen 1 in an area of Papua New Guinea in which malaria is holoendemic. Infect. Immunity 65, 5082-5087.
Krzych et al., 1995. T lymphocytes from volunteers immunized with irradiated Plasmodium falciparum sporozoites recognized liver and blood stage malaria antigens. J. Immunol. 155, 4072-4077.

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