Expression, processing and secretion of heterologous protein by

Details Expression, processing and secretion of heterologous protein by Expression, processing and secretion of heterologous protein by

1982-11-01

1988-10-04

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 70, 435 91, 4351721, 4351723, 435255, 435256, 435320, 935 28, 935 37, 935 47, 935 48, 935 50, 935 69, C12P 2100, C12P 2102, C12P 1934, C12N 1500, C12N 116, C12N 118

Patent

active

047756225

ABSTRACT:
Yeast organisms are caused to express, process, and secrete protein that is normally heterologous to yeast and not required for its viability by transforming yeast with an expression vector containing a gene encoding heterologous protein and a signal sequence also heterologous to yeast.

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Goeddel et al: Nature, 290, 20 (1981).
Sussman et al, "Pregrowth Hormone: Product of the Translation in Vitro of Messenger RNA Coding for Growth Hormone", Proc. Natl. Acad. Sci., U.S.A., 73: 29 (1976),
Webster's New Collegiate Dictionary, 1979, G. & C. Merriam Co., Springfield, Mass., p. 533.
Beach et al., "Nature", 290: 140-142 (12 Mar. 1981).
Taniguchi et al., "Proc. Natl. Acad. Sci. U.S.A.", 77(9): 5230-5233 (Sep. 1980).
Schekman et al. in The Molecular Biology of the Yeast Saccharomyces, Metabolism and Gene Expression, Strathern et al. (eds.), Cold Spring Harbor Laboratory, 1982, pp. 361-398.

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