Expression plasmids for improved production of heterologous prot

Details Expression plasmids for improved production of heterologous prot Expression plasmids for improved production of heterologous prot

1984-10-20

1990-06-19

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 694, 4351723, 435320, 935 27, 935 45, 935 73, C12N 120, C12N 1500, C12P 2100

Patent

active

049353705

ABSTRACT:
Promoter-ribosome binding site (rbs) expression elements of general utility for high level heterologous gene expression; plasmids carrying said promoter-rbs expression elements and encoding genetic information for direct high level expression in bacteria of heterologous proteins, especially plasmids carrying a gene coding for prorennin or mammalian growth hormones; methods for their construction, including the use of synthetic linkers to provide desirable functional properties thereto; recombinant microorganisms comprising said plasmids; expression of said bacterial produced heterologous proteins by said recombinant microorganisms; and demonstration of the activities of the thus-produced proteins.

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