Expression plasmid, a fusion protein, a transfected eukaryotic c

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 691, 435 698, 435 696, C12P 2102

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061141461

ABSTRACT:
The invention describes an expression plasmid containing a dicistronic transcription/translation unit, which unit comprises a sequence for a foreign protein and a sequence for a fusion protein, the fusion protein containing at least one selection marker and at least one amplification marker. Further described is a method of producing foreign proteins by using the plasmids according to the invention, as well as cell lines transformed with the plasmid according to the invention.

REFERENCES:
patent: 4775624 (1988-10-01), Bang et al.
patent: 5013653 (1991-05-01), Huston et al.
patent: 5171844 (1992-12-01), Van Ooyen et al.
patent: 5238820 (1993-08-01), Kaufman
patent: 5519115 (1996-05-01), Mapelli et al.
patent: 5591827 (1997-01-01), Brakenhoff et al.
Germann et al., "Retroviral Transfer of a Chimeric Multidrug Resistance-Adenosine Deaminase Gene", FASEB Journal, No. 4, pp. 1501-1507 (1990).
Sugimoto et al., "Efficient Expression of Drug-Selectable Genes in Retroviral Vectors Under Control of an Internal Ribosome Entry Site", Biotechnology, vol. 12, No. 7, pp. 694-698, (1994).
Kaufman et al., "Expression, Purification, and Characterization of Recombinant Carboxylated Factor IX Synthesized in Chinese Hamster Ovary Cells", J. Bio. Chem., vol. 261, No. 21, pp. 9622-9628, (1986).
Southern et al., "Transformation of Mammalian Cells to Antibiotic Resistance with a Bacterial Gene Under Control of the SV40 Early Region Promoter", J. Molecular & Applied Genetics, vol. 1, pp. 327-341, (1982).
Blochlinger et al., "Hygromycin B Phosphotransferase as a Selectable Marker for DNA Transfer Experiments with Higher Eucaryotic Cells", Molecular and Cellular Biology, pp. 2929-2931, (1984).
McIvor et al., "Isolation & Characterization of a Variant Dihydrofolate Reductase cDNA from Methotrexate-Resistant Murine L5178Y Cells", Nucleic Acids Res., vol. 18, No. 23, pp. 7025-7032, (1990).
Kim et al., Stable Reduction of Thymidine Kinase Activity in Cells Expressing High Levels of Anti-Sense RNA, Cell, vol. 42, pp. 129-138, (1985).
Wirth et al., "Genetic Engineering of Animal Cells", Genetic Engineering of Animals, pp. 1-82, (1993).
Kaufman, "Selection and Coamplification of Heterologous Genes in Mammalian Cells", Gene Selection and Coamplification, Methods in Enzymology, vol. 185, pp. 537-566, (1990).
McCormick et al., "Inducible Expression of Amplified Human Beta Interferon Genes in CHO Cells", Molecular and Cellular Biology, pp. 166-172, (1984).
Haynes, et al., "Constitutive, Long-term Production of Human Interferons by Hamster Cells Containing Multiple copies of a Cloned Interferon Gene", Nucleic Acids Res., vol. 11, No. 3 (1983).
Onomichi et al., "Expression of Amplified cDNA & Genomic Sequence Encoding Human Interleukin 2 in Chinese Hamster Ovary Cells", J. Biochem., vol. 102, pp. 123-131, (1987).
Walls et al., "Amplification of Multicistronic Plasmids in the Human 293 Cell Line & Secretion of Correctly Processed Recombinant Human Protein C", Gene, vol. 81, pp. 139-149, (1989).
Wernicke et al., "Generation of Recombination CHO(dhfr-) Cell Lines by Single Selection for dhfr+ Transformants", Analytical Biochem., vol. 203, pp. 146-150, (1992).
Kaufman et al., "Translational Efficiency of Polycistronic mRNAs and Their Utilization to Express Hetero. Genes in Mammalian Cells", EMBO Journal, vol. 6, No. 1, pp. 187-193, (1987).
Kozak et al., "Effects of Intercistronic Length on the Efficiency of Reinitiation by Eurcaryotic Ribosomes", Molecular and Cellular Biology, pp. 3438-3445, (1987).
Pelletier et al., "Internal Initiation of Translation of Eukaryotic MRNA Directed by a Sequence Derived From Poliovirus RNA", Nature, vol. 334, pp. 320-325, (1988).
Jang et al., "Initiation of Protein Synthesis by Internal Entry of Ribosomes into the 5' Nontranslated Region of Encephalomyocarditis Virus RNA In Vivo", J. of Virology, pp. 1651-1660, (1989).
Kaufman et al., "Improved Vectors for Stable Expression of Foreign Genese in Mammalian Cells by Use of the Untranslated Leader Sequence From EMC Virus", Nucleic Acids Res., vol. 19, No. 16, pp. 4485-4490, (1991).
Schwartz et al., "A Dominant Positive and Negative Selectable Gene for Use in Mammalian Cells", Proc. Natl. Acad. Sci., vol. 88, pp. 10416-10420, (1991).
Jorgensen et al., "Expression of completely .gamma.-Carboxylated Recombinant Human Prothrombin", J. of Bio. Chem., vol. 262, No. 14, pp. 6729-6734, (1987).
Falkner et al., "High Level Expression of Active Human Prothrombin in a Vaccina Virus Expression System", Thrombosis and Haemostasis, vol. 68, No. 2, pp. 119-124, (1992).
Kaufman et al., "Synthesis, Processing, and Secretion of Recombinant Human Factor VIII Expressed in Mammalian Cells", J. of Bio. Chem., vol. 263, No. 13, pp. 6352-6362, (1988).
Eaton et al., "Construction and Characterization of an Active Factor VIII Variant Lacking the Central One-Third of the Molecule", Biochemistry, vol. 25, No. 26, pp. 8343-8347, (1986).
Mertens et al., "Biol. Acitiv. of Recombinant Factor VIII Vari. Lacking the Centr. B-Domain 7 Heavy-Chain Sequ. Lys713-Arg740: Discordant In Vitro & In Vivo Activity", Brit. J. Hematol., vol. 85, pp. 133-142, (1993).
Toole et al., "A Large Region (=95 kDa) of Human Factor VIII Dispensable for In Vitro Procoagulant Activity", Proc. Natl. Acad. Sci. USA, vol. 83, pp. 5939-5942, (1986).
Pittman et al., "Biochemical, Immunological, & In Vivo Funct. Characterization B-Domain-Deleted Factor VIII" Blood, vol. 81, No. 11, pp. 2925-2935, (1993).
Balland et al., "Characterization of Two Differently Processed Forms of Human Recombinant Factor IX Synthes. in CHO Cells Trans. With A Polycistronic Vector", Eur. J. Biochem., vol. 172, pp. 565-572, (1988).
Grinnell et al, "Native and Modified Recombinant Human Protein C: Function, Secretion, and Posttranslational Modifications", Adv. Appl. Biotech. Ser., pp. 29-63, (1990).
Urlaub et al., "Isolation of Chinese Hamster Cell Mutants Deficient In Dihydrofolate Reductase Activity", Proc. Natl. Acad. Sci. USA, vol. 77, No. 7, pp. 4216-4220, (1980).
MacGregor et al., "Construction of Plasmids That Express E. Coli B-Galactosidase In Mammalian Cells", Nucleic Acids Research, vol. 17, No. 6, p. 1, (1989).
Kaufman et al., "Construction of a Modular Dihydrofolate Reductase cDNA Gene: Analysis of Signals Utilized for Efficient Expression", Molecular & Cell. Biol., pp. 1304-1319, (1982).
Towbin et al., "Electrophoretic Transfer of Proteins From Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications", Proc. Natl. Acad. Sci. USA, vol. 76, No. 9, pp. 4350-4354, (1979).
Leyte et al., "Inhibition of Human Coagulation Factor VIII by Monoclonal Antibodies", Biochem. J., vol. 263, pp. 187-194, (1989).
Neumann et al., "Gene Transfer Into Mouse Lyoma Cells by Electroporation In High Electric Fields", EMBO Journal, vol. 1, No. 7, pp. 841-845, (1982).
Stel et al., "Charac. 25 Monoclonal Antibodies Fact. VIII-von Willebrand Fact.: Relat. Betw. Ristocetin-Induced Platelet Aggrega & Platelet Adherence Subendoth.", Blood, vol. 63, No. 6, pp. 1408-1416, (1984).
Schmike et al., "Chromosomal and Extrachromosomal Localization of Amplified Dihydrofolate Reductase Genes in Cultured Mammalian Cells", CSHSA, vol. 45, pp. 785-797, (1988).
Kaufman et al., "Evolution of Chromosomal Regions Containing Transfected and Amplified Dihydrofolate Reductase Sequences", Molecular and Cellular Biology, pp. 699-711, (1983).
Leyte et al., "Sulfation of Tyr.sup.1680 of Human Blood Coagulation Factor VIII Is Esential for the Interaction of Factor VIII with von Willebrand Factor", Journal of Bio. Chem., vol. 266, No. 2, pp. 740-746, (1991).
Gunning et al., "A Human B-Actin Expression Vector System Directs High-Level Accumulation of Antisense Transcripts", Proc. Natl. Acad. Sci. USA, vol. 84, pp. 4831-4835, (1987).
Fischer et al., "Structural Analysis of Recombinant von Willebrand Factor: Identification of Hetero- and Homo-Dimers", FEBS Letters 351, pp. 345-348, (1994).
Laemmli et al., "Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4", Nature, vol. 227, pp. 680-685, (1970).
Ehrlich et al., "Direct Expr

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