Expression of zebrafish bone morphogenetic protein 4

Details Expression of zebrafish bone morphogenetic protein 4 Expression of zebrafish bone morphogenetic protein 4

2003-07-01

2008-09-23

Guzo, David (Department: 1636)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S023100, C800S020000

Reexamination Certificate

active

07427677

ABSTRACT:
Embodiments of the invention generally provide isolated DNA molecules, tissue-specific expression sequences, and promoter and regulatory DNA sequences involved in the regulation of bone morphogenetic protein 4 (BMP4). More specifically, the invention relates to regulation of gene expression in a tissue-specific manner. In one aspect, the invention provides zebrafish BMP4 gene, its structural organization, its promoter, and proximal and distal regulatory regions. In another aspect, the invention provides methods for identifying potential compounds/agents, potential molecular regulators, and the expression pattern for the expression of BMP4 gene.

REFERENCES:
patent: 6071518 (2000-06-01), Petersen
patent: 6083690 (2000-07-01), Harris et al.
patent: 6159696 (2000-12-01), Dijkema et al.
patent: 6379961 (2002-04-01), Jessell et al.
patent: 6458944 (2002-10-01), Kawai et al.
patent: 6475735 (2002-11-01), Sugiura
GenBank accession No. AY156927. Direct submission on Sep. 30, 2002 by Shentu, H. and Hwang, S.-P.L.
Bayer et al. A transgene containing IacZ is expressed in primary sensory neurons in zebrafish. Development 115: 421-426. 1992.
Alexander, et al., “Screening Mosaic F1 Females for Mutations Affecting Zebrafish Heart Induction and Patterning”,Dev. Genet., 22:288-299 (1998).
Amsterdam et al., “The Aequorea Victoria green fluorescent protein can be used as a reporter in live zebrafish embryos”,Dev. Biol., 171:123-129 (1995).
Blader et al., “Cleavage of the BMP-4 Antagonist Chordin by Zebrafish Tolloid”,Science, 278:1937-1940 (1997).
Carvajal et al., “A BAC transgenic analysis of the Mrf4/Myf5 locus reveals interdigitated elements that control activation and maintenance of gene expression during muscle development”,Development, 128:1857-1868 (2001).
Chen et al., “Left-right pattern of cardiac BMP4 may drive asymmetry of the heart in zebrafish”,Development, 124:4373-4382 (1997).
Chin et al., “Bone morphogenetic protein-4 expression characterizes inductive boundaries in organs of developing zebrafish”,Dev. Genes. Evol., 202:107-114 (1997).
Culp et al., “High-frequency germ-line transmission of plasmid DNA sequences injected into fertilized zebrafish eggs”,Proc. Natl. Acad. Sci. USA, 88:7953-7957 (1991).
Donovan et al., “Rapid Purification of Bacteriophage λ DNA”,BioTechniques, 15:602:603 (1993).
Feng et al., “The mouse bone morphogenetic protein-4 gene: analysis of promoter utilization in fetal rat calvarial osteoblasts and regulation by COUP-TFI orphan receptor”,J. Biol. Chem., 270:28364-28373 (1995).
Fu et al., “Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus”,Nature Biotech., 16:253:257 (1998).
Gaussin et al., “Endocardial cushion and myocardial defects after cardiac myocyte-specific conditional deletion of the bone morphogenetic protein receptor ALK3”,Proc. Natl. Acad. Sci. USA, 99:2878-2883 (2002).
Gong, Z. and Hew, C., “Transgenic Fish in Aquaculture and Developmental Biology”,Curr. Topics Dev. Biol., 30:177-214 (1995).
Hammerschmidt et al., “Genetic analysis of dorsoventral pattern formation in the zebrafish: requirement of a BMP-like ventralizing activity and its dorsal repressor”,Genes. Dev., 10:2452-2461 (1996).
Hogan, B., “Bone morphogenic proteins in development”,Curr. Opin. Genet. Dev., 6:432-438 (1996a).
Hogan, B., “Bone morphogenic proteins: multifunctional regulators of vertebrate development”,Curr. Opin. Genet. Dev., 10:1580-1594 (1996b).
Hsiao et al., “Enhanced Expression and Stable Transmission of Transgenes Flanked by Inverted Terminal Repeats From Adeno-Associated Virus in Zebrafish”,Dev. Dynam., 220:323-336 (2001).
Hu, et al., “Structure and Function of the Developing Zebrafish Heart”,Anatom. Rec., 260:148-157 (2000).
Hwang et al., “The Zebrafish BMP4 Gene: Sequence Analysis and Expression Pattern During Embryonic Development”,DNA Cell Biol., 16:1003-1011 (1997).
Jones et al., “Involvement of Bone Morphogenetic Protein-4 (BMP-4) and Vgr-1 in morphogenesis and neurogenesis in the mouse”,Development, 111:531-542 (1991).
Ju et al., “Faithful Expression of Green Fluorescent Protein (GFP) in Transgenic Zebrafish Embryos Under Control of Zebrafish Gene Promoters”,Dev. Genet., 12:158-167 (1999).
Marini et al., “Presistence and Replication of Plasmid DNA Microinjected into Early Embryos ofXenopus laevis”, Dev. Biol., 127:421-434 (1988).
Martinez-Barbera et al., “Cloning and expression of three members of the zebrafish Bmp family: Bmp2a, Bmp2b and Bmp 4”,Gene, 198:53-59 (1977).
Mowbray et al., “Expression of BMP signalling pathway members in the developing zebrafish inner ear and lateral line”,Mechanisms of Development, 108:179-184 (2001).
Muller et al., “Intronic enchancers control expression of zebrafishsonic hedgehogin floor plate and notochord”,Development, 126:2103-2116 (1999).
Nikaido et al., “Conservation of BMP signaling in zebrafish mesoderm patterning”,Mechanisms of Development, 61:75-88 (1997).
Ozkaynak et al., “Osteogenic Protein-2”,J. Biol. Chem, 267:25220-25227 (1992).
Park et al., “Analysis of Upstream Elements in theHuC Promoter Leads to the Establishment of Transgenic Zebrafish with Fluorescent Neurons”,Dev. Bio., 227:279-293 (2000).
Schilling et al., “Regulation of Left-Right Asymmetries in the Zebrafish byShhandBMP4”,Dev. Bio., 210:277-289 (1999).
Schultheiss et al., “A role for bone morphogenetic proteins in the induction of cardiac myogenesis”,Genes&Dev., 11:451-462 (1997).
Shafizadeh et al., “Transfenic Zebrafish Expressing Green Fluorescent Protein”,Methods in Molecular Biology, 183:225-233, 1990.
Stuart et al., “Replication, integration and stable germ-line transmission of foreign sequences injected into early zebrafish embryos”,Development, 103:403-412 (1988).
Stuart et al., “Stable lines of transgenic zebrafish exhibit reproductible patterns of transgene expression”,Development, 109:577-584 (1990).
Van den Wijngaard et al., “Genomic Organization of the Human Bone Morphogenetic Protein-4 Gene: Molecular Basis for Multiple Transcripts”,Biochem. And BioPhys. Res. Comm., 219:789-794 (1996).
Yelon et al., “Patterning during organogenesis: genetic analysis of cardiac chamber formation”,Cell&Dev. Bio., 10:93-98 (1999).
NCBI database, AF056336, 2003.
NCBI database, NM—131342, 2003.

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