Expression of TPA in mammalian cells

Details Expression of TPA in mammalian cells Expression of TPA in mammalian cells

1987-09-17

1992-04-28

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 4352412, 4353201, 536 27, 935 34, 935 61, C12P 2102, C12N 1512, C12N 1585

Patent

active

051089092

ABSTRACT:
Improved expression of tPA in mammalian cells is achieved employing a promoter region functional in a mammalian cell with a DNA sequence coding for tPA, where the sequence is interrupted by at least one intron. Particularly, a viral promoter is employed in conjunction with a hybrid gene having portions of the coding sequence uninterrupted by introns as compared to the wild-type gene and coding sequences interrupted by introns or the wild-type gene or mutants thereof.
Plasmid pSV7tPA2I was deposited on Feb. 14, 1985 and given A.T.C.C. Accession No. 40163.

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